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Monoclonal antibodies to monkey brain choline acetyltransferase: production and immunohistochemistry
T Ichikawa1, I Ishida, Y Hirata
1Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neurosciences, Japan.
Neuroscience Letters
|October 16, 1987
Abstract:
Two monoclonal antibodies to monkey brain choline acetyltransferase (ChAT) were obtained. Immunoblot analysis indicated that both antibodies revealed two adjacent protein bands on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ChAT was demonstrated immunohistochemically by one of the antibodies in the motoneurons in the monkey brainstem and spinal cord. This antibody also revealed ChAT-positive terminal-like structures in the neuropils of lamina IX of the cervical spinal cord and interpeduncular nucleus.