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Updated: Oct 14, 2026
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Isolation and Culture of Mouse Cortical Astrocytes
Published on: January 19, 2013
Lipid changes of astrocytes from mouse cerebellum cultured in lipid-free chemically defined medium and in
Neuroscience Letters
|November 10, 1987
Abstract:
Astrocytes of mouse cerebellum were grown in chemically defined medium for 14 days, subcultivated and grown in serum-containing medium for up to 14 days. After 14 days in defined medium the cells showed fatty degeneration from which they recovered histologically and chemically after one week in serum-supplemented medium. At this point 99% of the cells were glial fibrillary acidic protein-positive and synthesized phosphatidylinositol, phosphatidylserine and bis(monoacylglycero)phosphate besides other phospholipids.
