3D high-precision melt electro written polycaprolactone modified with yeast derived peptides for wound healing
Mahta Mirzaei1, Gianina Dodi2, Ioannis Gardikiotis3
1Université libre de Bruxelles (ULB), École polytechnique de Bruxelles - BioMatter unit, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium; Centre for Food Chemistry and Technology, Ghent University Global Campus, 119-5 Songdomunhwa-Ro, Yeonsu-Gu, Incheon, South Korea; Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
This study developed anti-inflammatory yeast-derived peptide-functionalized scaffolds for skin wound healing. These modified scaffolds significantly accelerated the healing process in animal models.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Immunology
Background:
- Skin wound healing is a complex process often impaired by inflammation.
- Biomaterial scaffolds can support tissue regeneration, but enhancing their bioactivity is crucial.
- Yeast-derived peptides offer a potential source of therapeutic agents for wound care.
Purpose of the Study:
- To functionalize poly(ε-caprolactone) (PCL) melt electro written (MEW) scaffolds with a yeast-derived peptide for enhanced skin wound healing.
- To evaluate the efficacy of peptide-modified scaffolds in vitro and in vivo.
- To optimize peptide conjugation methods for improved scaffold performance.
Main Methods:
- Screening of 13 yeast-derived peptides for anti-inflammatory and cell-stimulating properties.
- Functionalization of PCL MEW scaffolds with the selected peptide (VLSTSFPPW, VW-9) using carbodiimide (CDI) and thiol chemistry, with and without plasma treatment.
- In vitro assays assessing fibroblast and macrophage interactions, proliferation, and collagen production.
- In vivo evaluation of full-thickness excisional wounds in rat models.
Main Results:
- The peptide VW-9 demonstrated significant anti-inflammatory effects and stimulated fibroblast activity.
- MEW scaffolds modified with CDI and thiol chemistry, combined with plasma treatment, showed superior cell penetration, adhesion, proliferation, and anti-inflammatory properties.
- Peptide-modified MEW scaffolds significantly accelerated skin wound healing in rats compared to controls.
Conclusions:
- Yeast-derived peptides can effectively functionalize PCL MEW scaffolds for skin wound healing applications.
- Plasma-treated CDI and thiol conjugation methods yield optimal results for peptide-scaffold interaction and biological activity.
- This study presents a promising strategy for developing advanced biomaterials for regenerative medicine and wound care.
