Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

1.9K
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sb(Ⅴ) has distinct cellular uptake route and cytotoxic mechanism from Sb(Ⅲ): Anion channel 2 and sulfhydryl binding.

Journal of hazardous materials·2025
Same author

A critical review on arsenic and antimony adsorption and transformation on mineral facets.

Journal of environmental sciences (China)·2025
Same author

Novel arsenate-respiring bacteria drive arsenic biogeochemical cycling in Tibetan geothermal springs revealed by DNA-SIP based metagenomics.

Journal of hazardous materials·2024
Same author

New insights into thallium(I) behaviors at birnessite surfaces: Effects of an organic buffer and goethite.

Journal of hazardous materials·2024
Same author

Fast <i>On-Site</i> Speciation and High Spatial Resolution Imaging of Labile Arsenic in Freshwater and Sediment Using the DGT-SERS Sensor.

Analytical chemistry·2024
Same author

Organic matter in geothermal springs and its association with the microbial community.

The Science of the total environment·2024

Related Experiment Video

Updated: Aug 5, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.7K

Enzyme-based electrochemical biosensor for antimonite detection in water.

Rui Pei1, Li Ye1, Chuanyong Jing2

  • 1Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China.

Biosensors & Bioelectronics
|March 26, 2023
PubMed
Summary

We developed a novel electrochemical biosensor using modified arsenite oxidase (AioAB) within ZIF-8 to specifically detect antimonite (SbIII). This enzyme modification enhances SbIII detection sensitivity and selectivity for environmental monitoring.

Keywords:
AntimoniteElectrochemical biosensorEnzymeMetal-organic frameworksSubstrate specificity

More Related Videos

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.4K
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

14.3K

Related Experiment Videos

Last Updated: Aug 5, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.7K
Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.4K
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

14.3K

Area of Science:

  • Environmental Science
  • Biochemistry
  • Materials Science

Background:

  • Antimonite (SbIII) is a prevalent environmental contaminant requiring sensitive detection methods.
  • Existing enzyme-based electrochemical (EC) biosensors face challenges due to the lack of SbIII-specific oxidizing enzymes.

Purpose of the Study:

  • To engineer the specificity of arsenite oxidase (AioAB) for SbIII detection.
  • To develop a novel EC biosensor for ultrasensitive, on-site antimonite detection.

Main Methods:

  • Modulating the spatial conformation of AioAB using the metal-organic framework ZIF-8.
  • Characterizing enzyme structural changes via Raman spectroscopy.
  • Constructing and evaluating the AioAB@ZIF-8 EC biosensor performance.

Main Results:

  • The AioAB@ZIF-8 biosensor demonstrated high substrate specificity for SbIII (12.8 s-1 μM-1) over AsIII (1.1 s-1 μM-1).
  • Structural analysis confirmed relaxed AioAB conformation within ZIF-8, involving S-S bond breakage and α-helix to random coil conversion.
  • The sensor achieved a dynamic linear range of 0.041-4.1 μM, a 5s response time, and a low detection limit of 0.041 μM with high sensitivity (1894 nA μM-1).

Conclusions:

  • Enzyme specificity can be tuned by altering spatial conformation, offering a new strategy for biosensing metal(loid)s.
  • The AioAB@ZIF-8 EC biosensor provides a promising platform for ultrasensitive and selective on-site detection of antimonite.