Highly selective chemosensor for the sensitive detection of Hg²⁺ in aqueous media and its cell imaging application

The deleterious toxicity of Hg²⁺ on ecological and biological system makes it crucial for the precise monitoring of Hg²⁺. Herein, we prepared a novel "turn-on" chemosensor N'-(4-(methylthio)butan-2-ylidene) rhodamine B hydrazide (denoted as MTRH) by a simple two-step reaction. MTRH exhibited an ultra-low detection limit (LOD) in fluorescence measurement of Hg²⁺ in pure aqueous media, which was estimated to be 1.3 × 10^-9 mol·L^-1. Moreover, the proposed chemosensor holds the ability of visualizing Hg²⁺ by the distinct color change of the solution. The corresponding recognition mechanism was investigated by Job's plots, mass spectrometry and DFT calculation analysis. Importantly, the characteristics such as high sensitivity, low cytotoxicity and good biocompatibility of MTRH exhibited in the application of detecting Hg²⁺ in real water sample and bioimaging of intracellular Hg²⁺ prove that MTRH is a promising tool to evaluate the levels of Hg²⁺ in complex biological systems.