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Related Experiment Video

Updated: Aug 5, 2025

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
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Visualizing multiciliated cells in the zebrafish.

Hannah M Wesselman1, Allison E Gatz1, Rebecca A Wingert1

  • 1Department of Biological Sciences, Center for Stem Cells and Regenerative Medicine, Center for Zebrafish Research, Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, Notre Dame, IN, United States.

Methods in Cell Biology
|March 26, 2023
PubMed
Summary
This summary is machine-generated.

Multiciliated cells (MCCs) are crucial for fluid movement in various organs. This study uses zebrafish to explore MCC development and function in the kidney, offering insights into ciliopathic diseases.

Keywords:
CiliaCiliopathiesDevelopmentKidneyMulticiliated cellNephronPronephrosZebrafish

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Physiology

Background:

  • Ciliated cells, particularly multiciliated cells (MCCs), are vital for sensing and fluid propulsion in systems like the respiratory and reproductive tracts.
  • The presence and role of MCCs in the kidney, especially during disease, remain poorly understood.
  • Ciliopathies, diseases linked to ciliary dysfunction, require better understanding of cilia development and function.

Purpose of the Study:

  • To investigate the development and characteristics of multiciliated cells (MCCs) in the zebrafish kidney.
  • To establish methods for visualizing and quantifying ciliated cells and their features in vivo.
  • To utilize the zebrafish as a model for studying conserved ciliogenesis pathways relevant to human kidney development and disease.

Main Methods:

  • Utilized the zebrafish (Danio rerio) model organism, focusing on its embryonic kidney (pronephros).
  • Developed and applied methods for visualizing ciliated cells and their specific features.
  • Quantified ciliated cell number and various cilia-related characteristics in the developing zebrafish kidney.

Main Results:

  • Successfully visualized and quantified ciliated cells, including MCCs, in the developing zebrafish kidney.
  • Established a tractable in vivo model for studying ciliogenesis and ciliated cell dynamics.
  • Provided a foundation for investigating the role of MCCs in kidney pathobiology.

Conclusions:

  • The zebrafish pronephros is a suitable model for studying ciliated cell development and ciliogenesis.
  • The developed methods enable detailed analysis of ciliated cell populations in vivo.
  • This research facilitates further investigation into the function of MCCs in kidney health and disease.