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Allosteric Regulation

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...

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Related Experiment Video

Updated: Aug 5, 2025

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

In vitro allosteric transcription factor-based biosensing.

Shanshan Li¹, Zilong Li², Gao-Yi Tan³

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.

Trends in Biotechnology

|March 26, 2023

Summary

This summary is machine-generated.

Bacterial allosteric transcription factors (aTFs) offer a novel approach for in vitro biosensing. This review highlights their advantages over whole-cell biosensors (WCBs) for detecting small molecules.

Keywords:

allosteric transcription factor biosensing recognition element small molecule

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Last Updated: Aug 5, 2025

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Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Biosensors convert biological responses into measurable signals.
Bacterial allosteric transcription factors (aTFs) are emerging as recognition elements for biosensing.
Whole-cell biosensors (WCBs) have limitations that in vitro approaches aim to overcome.

Purpose of the Study:

To review recent advances in configuring in vitro biosensors using aTFs.
To evaluate the advantages of aTFs for in vitro biosensing applications.
To highlight the potential of aTFs for developing robust and user-friendly biosensing strategies.

Main Methods:

Review of current literature on aTF-based in vitro biosensors.
Analysis of aTF properties relevant to biosensing.
Evaluation of strategies for constructing aTF-based biosensors.

Main Results:

aTFs offer a viable alternative to WCBs for in vitro biosensing.
aTFs provide advantages for detecting small molecules.
Recent technical innovations facilitate the development of diverse aTF biosensors.

Conclusions:

In vitro aTF biosensors overcome limitations of WCBs.
aTFs show great potential for robust and easily implemented biosensing.
Generalizable workflows and technical innovations will accelerate the development of aTF-based small-molecule biosensors.