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Related Experiment Video

Updated: Aug 5, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

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Efficient isolation of rare B cells using next-generation antigen barcoding.

Jonathan Hurtado1,2,3, Claudia Flynn1,3,4, Jeong Hyun Lee3,4,5

  • 1Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, United States.

Frontiers in Cellular and Infection Microbiology
|March 27, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a streamlined method for isolating antigen-specific B cells, accelerating the discovery of therapeutic monoclonal antibodies (mAbs) and advancing vaccine development. The approach efficiently identifies rare B cell precursors for broadly neutralizing HIV antibodies.

Keywords:
AIRRBCRantibodyhumoral immunologyimmunologymulti-omicssingle cell

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Area of Science:

  • Immunology
  • Biotechnology
  • Genomics

Background:

  • Discovering therapeutic monoclonal antibodies (mAbs) and developing vaccines is often costly and time-consuming.
  • Advances in single-cell genomics and emulsion microfluidics enable high-throughput processing of individual cells.
  • Efficient isolation of antigen-specific B cells is crucial for accelerating these discovery pipelines.

Purpose of the Study:

  • To present a streamlined method for the high-throughput isolation and analysis of antigen-specific B cells.
  • To demonstrate the utility of this method in discovering therapeutic monoclonal antibodies (mAbs).
  • To facilitate rational vaccine development by enabling the identification of key B cell precursors.

Main Methods:

  • Development of a streamlined workflow for B cell isolation and analysis.
  • Implementation of next-generation antigen barcoding techniques.
  • Integration of a computational framework for multi-omics analysis of B cells.

Main Results:

  • Successful isolation and analysis of a large number of antigen-specific B cells.
  • Recovery of thousands of antigen-specific monoclonal antibodies (mAbs).
  • Efficient isolation of rare B cell precursors for VRC01-class and IOMA-class broadly neutralizing HIV antibodies.

Conclusions:

  • The presented method significantly accelerates the discovery of therapeutic mAbs and aids in rational vaccine design.
  • This approach enables the efficient identification of rare B cell populations, including those targeting conserved epitopes.
  • The integrated computational framework enhances the analysis of multi-omics data from B cells.