Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:36

Mismatch Repair

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of chemokines in Escherichia coli.

Methods in molecular biology (Clifton, N.J.)·2000
Same author

Recombinant guinea pig and human RANTES activate macrophages but not eosinophils in the guinea pig.

Journal of immunology (Baltimore, Md. : 1950)·1997
Same author

Purification, characterization, and in vitro phosphorylation of the neuron-specific membrane-associated protein SCG10.

Protein expression and purification·1997
Same author

A bacterial signal peptide directs efficient secretion of eukaryotic proteins in the baculovirus expression system.

Protein expression and purification·1997
Same author

Structure and bioactivity of recombinant human CTAP-III and NAP-2.

Journal of protein chemistry·1997
Same author

Dissecting processing and apoptotic activity of a cysteine protease by mutant analysis.

The Journal of cell biology·1996

Related Experiment Video

Updated: Jun 26, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Mu insertion duplicates a 5 base pair sequence at the host inserted site.

B Allet

    Cell
    |January 1, 1979
    PubMed
    Summary

    Bacteriophage Mu lysogenization involves the duplication of five base pairs at the insertion site in the host DNA. This results in direct repeats flanking the Mu DNA, similar to insertion sequence (IS) element behavior.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Lysogenic bacteriophages integrate their DNA into the host genome.
    • Understanding the mechanism of bacteriophage Mu integration is crucial for molecular biology.
    • Previous studies have investigated DNA integration but lacked detailed sequence analysis at the insertion site.

    Purpose of the Study:

    • To analyze the nucleotide sequences at the integration site of lysogenic Mu DNA.
    • To determine if Mu DNA integration causes alterations in the host DNA sequence.
    • To compare the integration mechanism of Mu with other mobile genetic elements.

    Main Methods:

    • Analysis of nucleotide sequences at both ends of lysogenic Mu DNA.
    • Cloning of Mu DNA ends into lambda Mu hybrid particles.

    More Related Videos

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations
    04:52

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations

    Published on: February 3, 2023

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
    07:28

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

    Published on: January 10, 2025

    Related Experiment Videos

    Last Updated: Jun 26, 2026

    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
    08:19

    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

    Published on: July 7, 2020

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations
    04:52

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations

    Published on: February 3, 2023

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
    07:28

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

    Published on: January 10, 2025

  • Utilizing a Mu lysogen within the lacZ region of lambdaplac5 for analysis.

    • Main Results:

    • Mu lysogenization is associated with the duplication of 5 base pairs of lac DNA at the insertion site.
    • The integrated Mu DNA is flanked by two copies of these five base pairs, oriented as direct repeats.
    • Independent investigations confirmed these findings using a different Mu lysogen.

    Conclusions:

    • Bacteriophage Mu integration involves a 5 base pair duplication at the target site, creating direct repeats.
    • Mu DNA insertion mechanism shares similarities with insertion sequence (IS)-mediated DNA insertions.
    • The findings provide insights into the molecular mechanisms of viral DNA integration and genome evolution.