Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Z-DNA-binding proteins from bull testis.

S H Gut1, M Bischoff, R Hobi

  • 1Institut für Pharmakologie und Biochemie, Universität Zürich-Irchel, Switzerland.

Nucleic Acids Research
|December 10, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to the Letter Regarding "Neuroendocrine Deficits and Weight Development Before and After Proton Therapy in Children With Craniopharyngioma".

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Neuroendocrine Deficits and Weight Development Before and After Proton Therapy in Children With Craniopharyngioma.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Save the children by treating their mothers (PriVileG-M-study) - study protocol: a sequentially randomized controlled trial of individualized psychotherapy and telemedicine to reduce mental stress in pregnant women and young mothers and to improve Child's health.

BMC psychiatry·2019
Same author

Test characteristics of milk amyloid A ELISA, somatic cell count, and bacteriological culture for detection of intramammary pathogens that cause subclinical mastitis.

Journal of dairy science·2017
Same author

[Acetabular fractures in the elderly - primary endoprosthesis].

Der Orthopade·2017
Same author

Investigation of the refractive index repeatability for tantalum pentoxide coatings, prepared by physical vapor film deposition techniques.

Applied optics·2017

Researchers identified three Z-DNA-binding proteins from bull testis, crucial for understanding DNA structure and function in mammals. These proteins exhibit high specificity for Z-DNA, a non-B DNA conformation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Z-DNA is a left-handed helical conformation of DNA.
  • Z-DNA binding proteins play roles in DNA replication, transcription, and recombination.
  • Mammalian Z-DNA binding proteins are not well-characterized.

Purpose of the Study:

  • To isolate and characterize Z-DNA binding proteins from mammalian tissue.
  • To determine the binding affinity and specificity of these proteins for Z-DNA.

Main Methods:

  • Proteins were extracted from mature bull testis.
  • Purification involved sequential chromatography (cation exchange, anion exchange FPLC).
  • Z-DNA binding affinity was measured using Scatchard analysis with brominated poly(dG-dC).poly(dG-dC).

Related Experiment Videos

Main Results:

  • Three Z-DNA binding proteins (31, 33, and 58 kD) were purified to near homogeneity.
  • The 58 kD protein showed high affinity for Z-DNA (Kz = 6 X 10(-8) M), comparable to anti-Z-DNA antiserum.
  • All three proteins demonstrated high conformational specificity for Z-DNA over B-DNA.

Conclusions:

  • This study reports the first isolation of Z-DNA binding proteins from mammalian tissue.
  • These proteins are potential regulators of gene expression and DNA metabolism.
  • Further research is needed to elucidate their in vivo functions.