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Published on: March 30, 2019
The clinical significance and mechanism of microRNA-22-3p targeting TP53 in lung adenocarcinoma
Rui Lin1, Guo-Sheng Li2, Xiang-Yu Gan1
1Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Background:
At present, studies on MircoRNA-22-3p (miR-22-3p) in lung adenocarcinoma use a single method, lack multi-center validation and multi-method validation, and there is no big data concept to predict and validate target genes.
Objective:
To investigate the expression, potential targets and clinicopathological significance of miR-22-3p in lung adenocarcinoma (LUAD) tissues.
Methods:
LUAD formalin-fixed paraffin-embedded (FFPE) tumors and adjacent normal lung tissues were collected for real-time quantitative polymerase chain reaction (RT-qPCR). Collect miR-22-3p in LUAD and non-cancer lung tissue from high-throughput datasets, standardized mean difference (SMD) and area under the curve (AUC) of the comprehensive receiver operating curve (summary receiver operating characteristic cure, sROC curve) were calculated. Cell function experiments on A549 cells transfected with LV-hsa-miR-22-3p. Target genes were predicted by the miRwalk2.0 website and the resulting target genes were subjected to Gene Ontology (GO) pathway enrichment analysis and constructed to protein-protein interaction network. Finally, the protein expression level of the key gene TP53 was validated by searching The Human Protein Atlas (THPA) database to incorporate TP53 immunohistochemical results in LUAD.
Results:
RT-qPCR result from 41 pairs of LUAD and adjacent lung tissues showed that miR-22-3p was downregulated in LUAD (AUC = 0.6597, p= 0.0128). Globally, a total of 838 LUADs and 494 non-cancerous lung tissues were included, and were finally combined into 14 platforms. Compared with noncancerous tissue, miR-22-3p expression level was significantly reduced in LUAD tissue (SMD =-0.32, AUC = 0.72l); cell function experiments showed that miR-22-3p has inhibitory effects on cell proliferation, migration and invasion, and has promotion effect on apoptosis. Moreover, target genes prediction, GO pathway enrichment analysis and PPI network exhibited TP53 as a key gene of target gene of miR-22-3p; at last, a total of 114 high-throughput datasets were included, including 3897 LUADs and 2993 non-cancerous lung tissues, and were finally combined into 37 platforms. Compared with noncancerous tissue, TP53 expression level was significantly increased in LUAD (SMD = 0.39, p< 0.01) and it was verified by the protein expression data from THPA.
Conclusion:
Overexpression of miR-22-3p may inhibit LUAD cell proliferation, migration and invasion through TP53, and promote cell apoptosis.
Insights
MicroRNA-22-3p (miR-22-3p) is downregulated in lung adenocarcinoma (LUAD), inhibiting cancer cell growth and invasion. Overexpression of miR-22-3p may suppress LUAD progression by targeting TP53.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Current research on microRNA-22-3p (miR-22-3p) in lung adenocarcinoma (LUAD) lacks multi-center and multi-method validation.
- There is a need for big data approaches to predict and validate target genes for miR-22-3p in LUAD.
- Understanding miR-22-3p's role is crucial for developing novel LUAD diagnostics and therapeutics.
Purpose of the Study:
- To investigate the expression levels of miR-22-3p in LUAD tissues.
- To identify potential target genes of miR-22-3p.
- To determine the clinicopathological significance of miR-22-3p in LUAD.
Main Methods:
- Real-time quantitative polymerase chain reaction (RT-qPCR) was performed on LUAD and adjacent normal lung tissues.
- High-throughput datasets were analyzed to assess miR-22-3p expression in a large cohort of LUAD and non-cancerous lung tissues.
- Bioinformatic tools (miRwalk2.0, GO, PPI network) were used for target gene prediction and pathway analysis, with TP53 protein levels validated via The Human Protein Atlas (THPA).
Main Results:
- miR-22-3p was significantly downregulated in LUAD tissues compared to non-cancerous tissues (RT-qPCR: AUC=0.6597, p=0.0128; high-throughput data: SMD=-0.32, AUC=0.72).
- Functional experiments demonstrated that miR-22-3p inhibits LUAD cell proliferation, migration, and invasion, while promoting apoptosis.
- TP53 was identified as a key target gene of miR-22-3p, with significantly increased TP53 expression observed in LUAD tissues (SMD=0.39, p<0.01), validated at the protein level.
Conclusions:
- Downregulation of miR-22-3p is a significant finding in LUAD.
- miR-22-3p exerts tumor-suppressive effects by inhibiting proliferation, migration, and invasion.
- The findings suggest that miR-22-3p may inhibit LUAD progression through the TP53 pathway.
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