Shade-induced lncRNA PUAR promotes shade response by repressing PHYA expression.
Tongdan Zhu1, Chuanwei Yang1, Yu Xie1
1State Key Laboratory of Genetic Engineering, Institute of Plants Biology, School of Life Sciences, Fudan University, Shanghai, China.
EMBO Reports
|March 27, 2023
Summary
This study identifies novel long non-coding RNAs (lncRNAs) involved in plant shade avoidance. A specific lncRNA, PUAR, regulates PHYTOCHROME A (PHYA) expression to control shade-induced hypocotyl elongation.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Shade avoidance syndrome (SAS) is a critical plant response to vegetative shade, involving morphological and physiological adjustments to optimize light capture.
- Key regulators of SAS include positive regulators like PHYTOCHROME-INTERACTING 7 (PIF7) and negative regulators such as PHYTOCHROMES.
Purpose of the Study:
- To identify long non-coding RNAs (lncRNAs) regulated by shade conditions in Arabidopsis.
- To characterize the function and mechanism of a novel shade-induced lncRNA, PUAR, in regulating SAS.
Main Methods:
- Identification of shade-regulated lncRNAs in Arabidopsis using transcriptomic analysis.
- Characterization of PUAR, including its induction by shade and its physical interaction with PIF7.
- Analysis of PUAR's role in repressing PHYA gene expression and its impact on shade-induced hypocotyl elongation.
Main Results:
- 211 lncRNAs were identified as being regulated by shade in Arabidopsis.
- PUAR, a lncRNA from the PHYA locus, is induced by shade and promotes hypocotyl elongation.
- PUAR physically interacts with PIF7, inhibiting PIF7 binding to the PHYA 5' UTR and repressing PHYA induction.
Conclusions:
- lncRNAs play a significant role in the regulation of shade avoidance syndrome.
- PUAR acts as a novel regulator in SAS by modulating PHYA gene expression through interaction with PIF7.
- This study provides new mechanistic insights into lncRNA-mediated regulation of plant responses to shade.
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