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Updated: Aug 5, 2025
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
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Gas-Phase Behavior of Galactofuranosides upon Collisional Fragmentation: A Multistage High-Resolution Ion Mobility
Simon Ollivier1,2, Laurent Legentil3, Oznur Yeni4
1INRAE, UR BIA, F-44316 Nantes, France.
Journal of the American Society for Mass Spectrometry
|March 27, 2023
Summary
This study investigates the impact of carbohydrate tautomerism on gas-phase ion behavior using ion mobility spectrometry. Researchers found that furanose (Galf) containing oligosaccharides exhibit altered ion mobility profiles due to conformational changes.
Area of Science:
- Analytical Chemistry
- Carbohydrate Chemistry
- Structural Biology
Background:
- Carbohydrates are structurally diverse and challenging for analytical chemists.
- Monosaccharide units exist as pyranose (p) or furanose (f) ring forms, impacting biological properties.
- The gas-phase behavior of carbohydrate tautomers under ion mobility spectrometry (IMS) conditions is not well understood.
Purpose of the Study:
- To investigate the gas-phase behavior of furanose (Galf) containing oligosaccharides under collisional dissociation (CID) conditions.
- To analyze the impact of tautomerism on ion mobility profiles and fragmentation patterns.
- To explore the contributions of gas-phase conformers in Galf-containing oligosaccharides.
Main Methods:
- High-resolution and multistage ion mobility spectrometry (IMS) on a Cyclic IMS platform.
- Collisional dissociation (CID) of [M+Li]+ ions.
- Molecular dynamics simulations.
Main Results:
- Disaccharidic fragments from Galf-containing trisaccharides showed altered IMS profiles compared to standards.
- Evidence of Galf migration and other unidentified alterations in IMS profiles was observed.
- Multistage IMS and molecular dynamics revealed additional gas-phase conformers in Galf-containing fragments.
Conclusions:
- Carbohydrate tautomerism significantly influences gas-phase ion behavior and IMS profiles.
- Galf-containing oligosaccharides exhibit unique conformational dynamics in the gas phase.
- Further investigation into tautomerism is crucial for accurate carbohydrate structural characterization.
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