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Related Experiment Video

Updated: Aug 5, 2025

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
08:02

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Published on: March 24, 2023

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Mouse Models for Atopic Dermatitis.

Keiko Sakamoto1,2, Keisuke Nagao1

  • 1Cutaneous Leukocyte Biology Section, Dermatology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland.

Current Protocols
|March 27, 2023
PubMed
Summary

This study details a versatile mouse model for atopic dermatitis (AD) using topical MC903 (calcipotriol) to induce inflammation. Protocols are provided for assessing skin phenotypes, immune cell infiltration, and inflammation using flow cytometry and microscopy.

Keywords:
MC903atopic dermatitisflow cytometryhistological analysis

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Area of Science:

  • Immunology
  • Dermatology
  • Animal Models

Background:

  • Atopic dermatitis (AD) is a complex skin condition characterized by barrier dysfunction, inflammation, and itching.
  • Mouse models are crucial for understanding AD pathophysiology.
  • The MC903 (calcipotriol) topical application model offers a versatile method for inducing AD-like inflammation in various mouse strains.

Purpose of the Study:

  • To provide standardized protocols for inducing and assessing AD-like inflammation in mice using topical MC903.
  • To detail methods for evaluating skin phenotypes, immune cell infiltration, and inflammatory markers.

Main Methods:

  • Topical application of MC903 to induce AD-like skin inflammation.
  • Gross phenotype assessment of skin lesions.
  • Skin processing for flow cytometry to analyze immune cell populations.
  • Histologic and immunofluorescence microscopy for detailed analysis of inflammation and immune cell localization.

Main Results:

  • The MC903 model allows for reproducible induction of AD-like inflammation.
  • Combined analyses (flow cytometry, histology, immunofluorescence) enable comprehensive characterization of the inflammatory response.
  • Accurate assessment of inflammation degree, inflammatory infiltrate type, and immune cell localization is achievable.

Conclusions:

  • The MC903-induced mouse model is a valuable tool for studying AD pathogenesis.
  • Standardized protocols facilitate robust immunologic and morphologic investigations of AD.
  • This model aids in understanding the complex immune mechanisms underlying atopic dermatitis.