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Internal Normal Mode Analysis Applied to RNA Flexibility and Conformational Changes
Afra Sabei1, Talissa Gabriele Caldas Baia1, Raphaël Saffar1
1Université Paris Cité, CiTCoM, CNRS, F-75006 Paris, France.
Abstract:
We investigated the capability of internal normal modes to reproduce RNA flexibility and predict observed RNA conformational changes and, notably, those induced by the formation of RNA-protein and RNA-ligand complexes. Here, we extended our iNMA approach developed for proteins to study RNA molecules using a simplified representation of the RNA structure and its potential energy. Three data sets were also created to investigate different aspects. Despite all the approximations, our study shows that iNMA is a suitable method to take into account RNA flexibility and describe its conformational changes opening the route to its applicability in any integrative approach where these properties are crucial.
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