Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Proteomics01:33

Proteomics

7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.6K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.8K
3.8K
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

3.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Provider attitudes, practices, and barriers in alcohol-associated liver disease care: Findings from the CASL ALD SIG national survey.

Canadian liver journal·2026
Same author

Dissecting genetic variance structure and evaluating genomic prediction models for single-cross hybrids derived from Stiff Stalk and Non-Stiff Stalk maize heterotic groups.

G3 (Bethesda, Md.)·2026
Same author

Author Correction: Posttranslational modifications remodel proteome-wide ligandability.

Nature chemical biology·2026
Same author

Posttranslational modifications remodel proteome-wide ligandability.

Nature chemical biology·2026
Same author

Improving the quality of paracentesis practices in people with advanced cirrhosis in an ambulatory care setting.

Journal of the Canadian Association of Gastroenterology·2026
Same author

Galectin-3-Induced Glycan Lattices as Biophysical Modulators of Membrane Phase Behavior in Live Cells.

ACS chemical biology·2026
Same journal

Kinetic and Spatial Resolution by dSTORM of miniSOG-Mediated Proximity Labeling in Mammalian Cells.

Chembiochem : a European journal of chemical biology·2026
Same journal

Trylons: Polyamide Surrogate Substrates Enable High-Throughput Screening of Nylon-Degrading Enzymes.

Chembiochem : a European journal of chemical biology·2026
Same journal

Membrane-Associated Biomolecules for Synthetic Cell Signalling.

Chembiochem : a European journal of chemical biology·2026
Same journal

Hybrid Aminoferrocenes With Nitrate Esters for In Situ Peroxynitrite Generation: Complex Interplay Between Redox Chemistry, NO-Donor Reactivity, and Aminoferrocene Activation Influences In Vitro Anticancer Activity.

Chembiochem : a European journal of chemical biology·2026
Same journal

Towards the Enzymatic Labeling of Oligonucleotides With Triantennary N-Acetyl Galactosamine.

Chembiochem : a European journal of chemical biology·2026
Same journal

Structure and Activity of Class II Lanthipeptides From a Thermophilic Bacterium.

Chembiochem : a European journal of chemical biology·2026
See all related articles

Related Experiment Video

Updated: Aug 5, 2025

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.3K

Surfaceome Profiling Identifies Basigin-Chaperoned Protein Clients.

Zak Vilen1,2, Eugene Joeh1,2, Elizabeth Lee1,2

  • 1Skaggs Graduate School of Chemical and Biological Sciences, Scripps Research, 10550 N., Torrey, Pines Rd., La Jolla, CA, 92037, USA.

Chembiochem : a European Journal of Chemical Biology
|March 27, 2023
PubMed
Summary
This summary is machine-generated.

Basigin (Bsg) protein loss reduces cell surface expression of monocarboxylate transporters MCT1 and MCT4 in liver cells. This study highlights a new method for identifying cell surface chaperone protein clients.

Keywords:
cell surfacechaperoneproteomicssurfaceometrafficking

More Related Videos

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.1K
Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

11.3K

Related Experiment Videos

Last Updated: Aug 5, 2025

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.3K
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.1K
Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

11.3K

Area of Science:

  • Cell Biology
  • Proteomics
  • Biochemistry

Background:

  • The cell surface proteome (surfaceome) is crucial for cell interactions and communication.
  • Basigin (Bsg) is a glycoprotein that chaperones proteins to the cell surface, but its clients are not fully understood.
  • Non-canonical trafficking pathways can complicate the prediction of surface protein localization.

Purpose of the Study:

  • To identify proteins whose cell surface expression depends on Basigin (Bsg).
  • To investigate changes in the hepatic stellate cell surface proteome upon Bsg loss.
  • To establish the utility of surfaceome proximity labeling for discovering chaperone-protein interactions.

Main Methods:

  • Applied surfaceome proximity labeling integrated with quantitative mass spectrometry-based proteomics.
  • Analyzed changes in the surfaceome of hepatic stellate cells lacking Basigin (Bsg).
  • Compared Bsg's client identification with that of a related protein, neuroplastin (Nptn).

Main Results:

  • Loss of Basigin (Bsg) resulted in decreased cell surface expression of monocarboxylate transporters MCT1 and MCT4.
  • The identified Bsg-MCT1/MCT4 relationship was specific to Bsg and not observed with neuroplastin (Nptn).
  • The surfaceome proximity labeling method successfully identified specific clients of Bsg.

Conclusions:

  • Surfaceome proximity labeling is a powerful tool for identifying cell surface chaperone clients.
  • Basigin (Bsg) plays a role in the cell surface expression of MCT1 and MCT4 in hepatic stellate cells.
  • This method advances the understanding of cell surface protein trafficking and regulation.