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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

460
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
460

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Persistent Luminescence Zn2GeO4:Mn2+ Nanoparticles Functionalized with Polyacrylic Acid: One-Pot Synthesis and

Roxana M Calderón-Olvera1, Encarnación Arroyo1, Aaron M Jankelow2,3

  • 1Instituto de Ciencia de Materiales de Sevilla (CSIC-US), c/Américo Vespucio, 49, Seville 41092, Spain.

ACS Applied Materials & Interfaces
|March 27, 2023
PubMed
Summary

This study developed uniform zinc germanate nanoparticles (Zn2GeO4:Mn2+) using a microwave-assisted method with polyacrylic acid. These nanoparticles exhibit excellent stability and luminescence, enabling successful application in a sensitive immunoassay for detecting interleukin-6 in human serum and plasma.

Keywords:
biosensingcolloidal stabilityfunctionalizationimmunoassaynanoparticlespersistent luminescencephotostabilityzinc germanate

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Persistent luminescence phosphors like zinc germanate doped with Mn2+ (Zn2GeO4:Mn2+) show promise for biosensing and bioimaging.
  • Current limitations include challenges in achieving uniform size, shape, aqueous dispersibility, and surface functionalization of nanoparticulated phosphors.

Purpose of the Study:

  • To develop a method for synthesizing uniform Zn2GeO4:Mn2+ nanoparticles (NPs) with properties suitable for biosensing.
  • To characterize the NPs and optimize Mn2+ doping for luminescence.
  • To demonstrate the application of these NPs in a biosensing assay.

Main Methods:

  • A one-pot, microwave-assisted hydrothermal synthesis was employed using polyacrylic acid (PAA) as an additive to control NP formation and surface properties.
  • Comprehensive characterization techniques were used to analyze NP morphology, stability, and luminescence.
  • A persistent luminescence-based sandwich immunoassay was developed for detecting interleukin-6.

Main Results:

  • Uniform Zn2GeO4:Mn2+ nanoparticles were successfully synthesized, with PAA ensuring ordered aggregation and providing surface carboxylate groups for functionalization.
  • The NPs demonstrated high colloidal stability in aqueous media and chemical stability in phosphate-buffered saline.
  • Optimal Mn2+ doping levels were identified for photoluminescence (2.50% Mn) and persistent luminescence (0.50% Mn).
  • The Zn2GeO4:0.50%Mn2+ NPs were photostable and effectively used in an immunoassay for detecting interleukin-6 in undiluted human serum and plasma.

Conclusions:

  • The developed microwave-assisted hydrothermal method effectively produces uniform Zn2GeO4:Mn2+ nanoparticles with desirable properties for biosensing.
  • PAA plays a crucial role in NP uniformity, stability, and surface functionalization.
  • These Mn-doped Zn2GeO4 nanophosphors are promising candidates for advanced autofluorescence-free biosensing applications.