Facile preparation of a cost-effective platform based on ZnFe₂O₄ nanomaterials for electrochemical cell detection

Circulating tumor cells (CTCs) are important tumor markers that indicate early metastasis, tumor recurrence, and treatment efficacy. To identify and separate these cells from the blood, new nanomaterials need to be developed. The present study explored the potential application of ZnFe₂O₄ magnetic nanoparticles in capturing CTCs with cell surface markers. Folic acid was coupled to L-cysteine-capped ZnFe₂O₄ nanoparticles (ZC) to provide binding sites on ZnFe₂O₄ nanoparticles for the recognition of folate bioreceptors, which are highly expressed in MCF-7 breast cancer cells. The cytotoxicity of ZnFe₂O₄ nanoparticles and ZC against MCF-7 was analyzed with the MTT assay. After 24 h of incubation, there were IC50 values of 702.6 and 805.5 µg/mL for ZnFe₂O₄ and ZC, respectively. However, after 48 h of incubation, IC50 values of ZnFe₂O₄ and ZC were reduced to 267.3 and 389.7 µg/mL, respectively. The cell quantification was conducted with magnetically collected cells placed on a glassy carbon electrode, and the differential pulse voltammetry (DPV) responses were analyzed. This cost-effective ZnFe₂O₄-based biosensing platform allowed cancer cell detection with a limit of detection of 3 cells/mL, ranging from 25 to 10⁴ cells/mL. In future, these functionalized zinc ferrites may be used in electrochemical cell detection and targeted cancer therapy.