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Porphyrin-induced photodamage to isolated epidermal cells from hairless mice
S Sandberg1, T Bjørndal, O D Laerum
1Laboratory of Clinical Biochemistry, Gade Institute, Haukeland Hospital, University of Bergen, Norway.
Abstract:
UVA light photodamages epidermal cells, as measured by inhibition of enzymatic activity and inhibition of respiration. This photodamage is greatly augmented in the presence of protoporphyrin as a photosensitizer. The increase in photodamage is greater for the cytosolic and mitochondrial enzymes lactate dehydrogenase and glutamate dehydrogenase and least for the lysosomal enzymes beta-glucuroninidase and N-acetyl-beta-glucosaminidase. Upon irradiation in the presence of protoporphyrin, N-acetyl-beta-glucosaminidase is released from the epidermal cells. These results may help to explain some features of the pathogenesis of erythropoietic protoporphyria.