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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Updated: Aug 5, 2025

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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[An improved paraffin embedding method for small core needle biopsy: technical introduction and evaluation].

Wan-Ling Chen1, Chao-Ji Shi, Min Wang

  • 1Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China.

Shanghai Kou Qiang Yi Xue = Shanghai Journal of Stomatology
|March 27, 2023
PubMed
Summary

A novel modified agar pre-embedding technique significantly reduces tissue dehydration time for small core needle biopsy specimens. This method ensures reliable histological morphology and molecular detection, proving valuable for clinical pathology.

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Area of Science:

  • Histopathology
  • Biotechnology
  • Molecular Diagnostics

Context:

  • Small tissue specimens, such as those from core needle biopsies, present challenges in histological processing.
  • Maintaining tissue integrity and enabling accurate molecular analysis are critical for accurate diagnosis.

Purpose:

  • To evaluate a modified agar pre-embedded paraffin embedding method for small core needle biopsy specimens.
  • To assess its impact on tissue integrity, histological morphology, and the detection of protein and DNA.

Summary:

  • The modified agar pre-embedding method, utilizing molded embedding molds, reduced tissue dehydration time from 12 hours to 3.5 hours compared to conventional paraffin embedding.
  • Histological morphology, immunohistochemistry (IHC), and DNA fluorescence in situ hybridization (FISH) results were reliable and comparable between methods.
  • This technique is easier to implement and promote than standard agar pre-embedding.

Impact:

  • The modified method meets clinical pathological diagnostic requirements for tissue processing.
  • It offers a reliable and efficient approach for the analysis of core needle biopsy specimens.
  • This technique is recommended for clinical application in oral mucosal squamous cell carcinoma diagnostics.