[Use of a diploid cell line for detecting the toxic components in medical immunobiological preparations]
Abstract:
Besides specific antigens medical immunobiological agents (MIBA) contain chemical compounds (formaldehyde, aluminium hydroxide and mercury salt, merthiolate) in permissible concentrations. Therefore, the investigation of MIBA and their components should involve methods studying the effect of chemical compounds on cells and their structural components. For this purpose WHO recommends to use cell cultures. The results obtained show that cell cultures (constant and diploid lines) allow the differentiation in the degree of toxicity of chemical compounds constituting MIBA. Merthiolate had the strongest irreversible lethal effect. The technique can prove useful for more accurate evaluation of toxicity in inactivated bacterial and viral vaccines as well as in serum preparations. Cell culture can be successfully used for the detection of toxic components in vaccines and serum drugs, with the final safety tested by their injection to animals.
Insights
Cell cultures effectively detect toxicity in medical immunobiological agents (MIBA). Merthiolate showed the highest toxicity, indicating cell culture
Area of Science:
- Toxicology
- Cell Biology
- Immunology
Background:
- Medical immunobiological agents (MIBA) contain chemical compounds like formaldehyde, aluminum hydroxide, mercury salt (merthiolate), and specific antigens.
- Assessing the toxicity of these chemical components is crucial for MIBA safety.
Purpose of the Study:
- To investigate the utility of cell cultures for evaluating the toxicity of chemical compounds found in MIBA.
- To differentiate the toxicity levels of various chemical components within MIBA.
Main Methods:
- Utilized constant and diploid cell lines for toxicity testing.
- Applied cell culture techniques as recommended by the World Health Organization (WHO).
Main Results:
- Cell cultures successfully differentiated the toxicity of chemical compounds present in MIBA.
- Merthiolate demonstrated the most potent irreversible lethal effect on cell cultures.
Conclusions:
- Cell culture techniques are effective for detecting toxic components in inactivated vaccines and serum preparations.
- This method allows for a more accurate toxicity evaluation of MIBA components.
- Final safety assessment can be confirmed through animal injection studies.
