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The Antimicrobial Activity of Micron-Thin Sol-Gel Films Loaded with Linezolid and Cefoxitin for Local Prevention of

Beatriz Toirac1, John Jairo Aguilera-Correa2,3, Aranzazu Mediero4

  • 1Materials Science and Engineering and Chemical Engineering Department, Carlos III University of Madrid, 28911 Madrid, Spain.

Gels (Basel, Switzerland)
|March 28, 2023
PubMed

Insights

New sol-gel coatings effectively prevent orthopedic prosthesis-related infections (OPRI) by inhibiting staphylococcal biofilm formation. These coatings loaded with antibiotics show promise for clinical use in preventing these challenging infections.

Area of Science:

  • Biomaterials Science
  • Infectious Diseases
  • Orthopedic Surgery

Background:

  • Orthopedic prosthesis-related infections (OPRI) pose significant clinical challenges, necessitating effective prevention strategies.
  • Local antibiotic delivery systems are crucial for combating OPRI, offering advantages over systemic treatments.
  • Sol-gel coatings provide a promising platform for sustained local drug delivery in orthopedic applications.

Purpose of the Study:

  • To evaluate a novel hybrid organic-inorganic sol-gel coating for its potential in preventing OPRI.
  • To assess the degradation kinetics and antibiotic release profiles of the sol-gel coatings.
  • To investigate the in vitro efficacy of the coatings against bacterial biofilm formation and their impact on osteoblast cell viability.

Main Methods:

  • Development of a hybrid organic-inorganic sol-gel coating incorporating organopolysiloxanes and organophosphite.
  • Loading of the sol-gel coatings with varying concentrations of linezolid and/or cefoxitin.
  • In vitro assessment of coating degradation, antibiotic release kinetics, biofilm inhibition against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli, and MC3T3-E1 osteoblast cell viability.

Main Results:

  • The sol-gel coatings demonstrated controlled degradation and antibiotic release.
  • Significant inhibition of biofilm formation was observed against Staphylococcus aureus and Staphylococcus epidermidis.
  • No inhibition of Escherichia coli biofilm was achieved, but a synergistic effect of dual antibiotics was noted against Staphylococcus aureus. Osteoblast cell viability and proliferation were not compromised.

Conclusions:

  • The developed sol-gel coatings show potential as an innovative therapeutic strategy for preventing staphylococcal OPRI.
  • The coatings effectively inhibit biofilm formation by key staphylococcal species relevant to orthopedic infections.
  • Further clinical investigation is warranted to establish the efficacy of these coatings in preventing orthopedic prosthesis-related infections.
Keywords:
Electrochemical Impedance SpectroscopyEscherichia coliProsthesis-Related InfectionsStaphylococcus aureusStaphylococcus epidermidiscefoxitinlinezolidnanogelssol–gel coating

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