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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing R- µCP and Sequential Nucleophilic Substitution

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A compound PCP scheme underlies sequential rosettes-based cell intercalation.

Yichi Xu¹, Yunsheng Cheng¹, Allison T Chen¹

¹Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA.

Development (Cambridge, England)

|March 28, 2023

Summary

This summary is machine-generated.

Planar cell polarity regulates sequential rosettes in C. elegans embryos, distinct from prior models. This involves a novel two-component polarity scheme with non-muscle myosin and LAT-1, impacting cell migration.

Keywords:

C. elegans embryo Cell migration Lineage Multicellular rosette Planar cell polarity

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Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

Sequential rosettes are a collective cell behavior mediating directional cell migration in the C. elegans embryo.
Planar cell polarity (PCP) pathways are crucial for coordinating cell behaviors during development.

Purpose of the Study:

To investigate the molecular mechanisms regulating sequential rosette formation in C. elegans.
To elucidate the role of planar cell polarity in this specific type of collective cell migration.

Main Methods:

Utilized live imaging and genetic analysis in Caenorhabditis elegans embryos.
Examined the localization and function of key polarity proteins, including non-muscle myosin (NMY) and Van Gogh (VANG-1).
Investigated the role of LAT-1/Latrophilin in rosette formation and cell migration.

Main Results:

Demonstrated a distinct PCP-based polarity scheme regulating sequential rosettes, differing from established models.
Showed that non-muscle myosin (NMY) localization and edge contraction are perpendicular to Van Gogh localization.
Identified a two-component polarity scheme involving canonical PCP proteins and NMY-2, with LAT-1/Latrophilin essential for NMY-2 function.

Conclusions:

Established a novel mode of PCP-mediated cell intercalation during sequential rosette formation.
Highlighted the versatile nature of the PCP pathway in regulating diverse collective cell behaviors.
Revealed a previously unknown role for LAT-1/Latrophilin in multicellular rosette regulation.