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Related Experiment Video

Updated: Aug 5, 2025

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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COMMD10 Is Essential for Neural Plate Development during Embryogenesis.

Khanh P Phan1, Panayiotis Pelargos1, Alla V Tsytsykova1

  • 1Department of Neurosurgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Journal of Developmental Biology
|March 28, 2023
PubMed
Summary

COMMD10 protein is essential for embryonic development. Commd10 knockout mice embryos exhibit neural crest failure and developmental arrest by embryonic day 8.5, indicating COMMD10

Keywords:
COMMD10Sox10embryonic developmentneural crest

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Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Cellular Biology

Background:

  • The COMMD protein family plays roles in cellular processes like copper homeostasis and trafficking.
  • COMMD10's specific function in embryonic development remains largely uncharacterized.

Purpose of the Study:

  • To investigate the role of COMMD10 in embryonic development using a mouse model.
  • To elucidate the molecular mechanisms underlying COMMD10's function during embryogenesis.

Main Methods:

  • Generation of a functional COMMD10 knockout mouse model (Commd10/J mice) using Vav1-cre transgene.
  • Phenotypic analysis of Commd10-deficient embryos at embryonic day 8.5 (E8.5).
  • Transcriptome analysis (RNA sequencing) to compare gene expression in mutant versus wild-type embryos.

Main Results:

  • Commd10 knockout embryos exhibited developmental arrest by E8.5, with no viable offspring.
  • Reduced expression of neural crest-specific genes, including the key regulator Sox10, was observed in mutant embryos.
  • Upregulation of genes associated with tissue remodeling and regression was noted in Commd10-deficient embryos.

Conclusions:

  • COMMD10 is crucial for embryonic survival, with its absence leading to neural crest failure.
  • COMMD10 plays a critical role in regulating gene expression essential for neural crest development and early embryogenesis.
  • This study reveals a novel function for COMMD10 in neural development.