Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

63
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
63
Viruses of Archaea01:29

Viruses of Archaea

42
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
42

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of lymphocytic choriomeningitis virus (LCMV) in the common house mice (<i>Mus musculus</i>) in Italy: an underrecognized threat to human health.

Microbiology spectrum·2026
Same author

Exploratory Metaviromic Analysis of the Sea-Rock Pool Mosquito <i>Aedes mariae</i> and the Water of Its Breeding Habitat.

Biology·2026
Same author

Feline bocaparvovirus in domestic cats with gastrointestinal disease.

Veterinary journal (London, England : 1997)·2026
Same author

Exploring Antibiotic Resistance Patterns in Isolates from Domestic Animals in Southern Italy.

Antibiotics (Basel, Switzerland)·2026
Same author

Multidimensional Analysis of SARS-CoV-2 RNA in Nine Sites Located in Campania Region, Italy.

Microorganisms·2026
Same author

Development and Qualification of a Pseudotyped Virus-Based Microneutralisation Assay for Influenza D Virus.

Influenza and other respiratory viruses·2026

Related Experiment Video

Updated: Aug 5, 2025

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.9K

Exploring the Astrovirome of Shellfish Matrices Using Nanopore Sequencing.

Farzad Beikpour1, Francesco Pellegrini1, Gianvito Lanave1

  • 1Department of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, Italy.

Veterinary Sciences
|March 28, 2023
PubMed
Summary

Researchers explored the astrovirus diversity in shellfish using nanopore sequencing. They discovered numerous novel astrovirus sequences, primarily of avian origin, highlighting potential contamination risks in marine environments.

Keywords:
astrovirusentericshellfishvirome

More Related Videos

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.2K
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.8K

Related Experiment Videos

Last Updated: Aug 5, 2025

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.9K
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.2K
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.8K

Area of Science:

  • Virology
  • Environmental Microbiology
  • Molecular Biology

Background:

  • Astroviruses are significant enteric pathogens found across diverse hosts, including humans, mammals, birds, and invertebrates.
  • The genetic variability of astroviruses complicates diagnostic approaches and taxonomic classification.
  • Filter-feeding mollusks can act as reservoirs and indicators of aquatic viral contamination.

Purpose of the Study:

  • To investigate the presence and diversity of astroviruses in filter-feeding mollusks.
  • To demonstrate the utility of nanopore sequencing for astrovirome characterization.
  • To identify novel astrovirus strains and understand their ecological significance.

Main Methods:

  • Utilized a pan-astrovirus consensus primer set for nested RT-PCR to amplify RNA-dependent RNA polymerase (RdRp) gene fragments.
  • Employed nanopore sequencing for deep sequencing of amplicons from bivalve samples.
  • Analyzed sequence data to identify and characterize astrovirus RdRp sequences.

Main Results:

  • Identified a wide array of known and unknown astrovirus RdRp sequence types in mollusks, many distantly related to existing database entries.
  • Generated 37 distinct astrovirus sequence contigs from various bivalve samples.
  • Predominant sequences were of avian origin, suggesting marine bird contamination of shellfish harvesting waters; aquatic astroviruses were also detected, but human astroviruses were absent.

Conclusions:

  • Nanopore sequencing coupled with consensus primers is effective for exploring the astrovirome in mollusks.
  • Mollusks harbor diverse astroviruses, with avian strains being a significant component, indicating potential environmental contamination pathways.
  • Further research is needed to understand the full spectrum and implications of astroviruses in aquatic ecosystems.