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Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

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Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
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Updated: Aug 5, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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Allosteric Nucleic Acid Enzyme: A Versatile Stimuli-Responsive Tool for Molecular Computing and Biosensing

Yuqiang Hu1,2, Changjiang Li1, Minghao Hu1

  • 1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)
|March 29, 2023
PubMed
Summary

This study introduces a novel allosteric nucleic acid enzyme (ANAzyme) for precise control of biomolecule function. This versatile DNAzyme platform enables new molecular computing and biosensing applications.

Keywords:
DNA circuitsallosteric regulationcoregulatory nanodevicesdeoxyribozymeeffectors

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Area of Science:

  • Biochemistry and Molecular Biology
  • Synthetic Biology
  • Nanotechnology

Background:

  • Allostery regulates biomolecule function via effector binding.
  • Deoxyribozymes (DNAzymes) offer catalytic and recognition capabilities but face regulatory challenges.
  • Existing DNAzyme regulations suffer from activity decay, signal leakage, and limited effector response.

Purpose of the Study:

  • To establish a rational regulation strategy for versatile effector-responsive allosteric nucleic acid enzymes (ANAzymes).
  • To overcome limitations of current DNAzyme regulation strategies.
  • To demonstrate the potential of ANAzymes in molecular computing and other applications.

Main Methods:

  • Engineered ANAzymes with an allosteric domain for diverse effector response.
  • Investigated predictable and fine modulation of enzyme-like activity.
  • Constructed allosterically coregulatory nanodevices, logic gates, and circuits.

Main Results:

  • Developed a versatile ANAzyme strategy with improved effector responsiveness.
  • Achieved predictable and fine-tuned modulation of catalytic activity.
  • Successfully built nanodevices, logic gates, and circuits using the ANAzyme system.

Conclusions:

  • The proposed ANAzyme strategy offers a significant advancement over previous DNAzyme regulation methods.
  • This rational design expands the toolbox for stimuli-responsive allosteric DNA materials.
  • ANAzymes show great potential for molecular computing, biosensing, and gene-silencing tools.