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Prenatal Restraint Stress-Induced Maternal Exposure and Clomifene Citrate Administration Modulates Reproductive
Pakistan Journal of Biological Sciences : PJBS
|March 29, 2023
Summary
Restraint stress significantly impairs female rat reproductive health by altering hormones and reducing litter size. Clomiphene citrate partially counteracts these stress-induced effects on fertility.
Area of Science:
- Reproductive biology
- Endocrinology
- Stress physiology
Background:
- Infertility and maternal stress are significant public health concerns globally.
- Understanding the interplay between stress and reproductive function is crucial.
Purpose of the Study:
- To investigate the effects of restraint stress on reproductive programming in Wistar rats.
- To evaluate the modulatory role of clomiphene citrate (CC) in stressed and unstressed rats.
Main Methods:
- 119 Wistar rats were subjected to daily restraint stress for 1-2 weeks.
- Hormonal assays (corticosterone, estrogen, progesterone, FSH, LH) were performed.
- Reproductive parameters including gestational length, litter size, and pup weight were assessed.
Main Results:
- Restraint stress elevated corticosterone and reduced reproductive hormones (estrogen, progesterone, FSH, LH).
- Stress significantly decreased gestation length and litter size; pup weight remained unaffected.
- Clomiphene citrate improved litter size in unstressed rats and partially restored it in stressed rats.
Conclusions:
- Stress negatively impacts female rat reproductive potential and hormonal balance.
- Stress diminishes the efficacy of clomiphene citrate in ovulation induction.
