Related Experiment Video

Updated: Aug 5, 2025

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
06:15

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Published on: December 20, 2018

7.5K

Cytotoxicity and Antimicrobial Resistance of Aeromonas Strains Isolated from Fresh Produce and Irrigation Water

Alberto Pintor-Cora1,2, Olga Tapia1, María Elexpuru-Zabaleta1

  • 1Research Group on Foods, Nutritional Biochemistry and Health, Universidad Europea del Atlántico, 39001 Santander, Spain.

Antibiotics (Basel, Switzerland)
|March 29, 2023
PubMed

Insights

Certain Aeromonas strains from produce and water show high virulence and cytotoxicity. These findings highlight potential health risks, emphasizing the need for further research on foodborne Aeromonas.

Area of Science:

  • Microbiology
  • Food Safety
  • Pathogen-Host Interactions

Background:

  • The genus *Aeromonas* is increasingly recognized for its presence in food safety contexts, with growing concerns about antibiotic resistance.
  • Understanding the pathogenic potential of *Aeromonas* strains is crucial for public health, especially those isolated from food and water sources.

Purpose of the Study:

  • To investigate the cytotoxic and pathogenic capabilities of *Aeromonas* strains isolated from fresh produce and water.
  • To analyze the interaction of these *Aeromonas* strains with various eukaryotic cell types and an invertebrate model.

Main Methods:

  • Utilized HT-29, Vero, J774.1 cells, and primary mouse embryonic fibroblasts to assess cytotoxicity.
  • Employed confocal microscopy for analyzing host-cell pathogen interactions.
  • Assessed pathogenicity using the *Galleria mellonella* larval model.

Main Results:

  • Two *Aeromonas* strains exhibited significant cytotoxicity against epithelial cells, fibroblasts, and macrophages.
  • These highly cytotoxic strains also demonstrated high virulence in the *Galleria mellonella* model.
  • Most tested *Aeromonas* strains displayed low resistance to various antibiotic families.

Conclusions:

  • Specific *Aeromonas* strains found in vegetables and water possess considerable in vitro and in vivo virulence.
  • These findings underscore a potential health hazard associated with contaminated food and water.
  • Further investigation into the significance of foodborne *Aeromonas* is warranted, particularly in light of emerging antimicrobial resistance concerns.
Keywords:
Aeromonasantimicrobial resistancecytotoxicityhost–pathogen interactionsvirulence

More Related Videos

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

6.6K
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems09:58

Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems

Published on: December 8, 2017

9.9K

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
102
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
53
Antibiotic Selection00:57

Antibiotic Selection

Overview
54.9K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
52
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.2K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
148