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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Analyzers: Overview01:13

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Updated: Aug 5, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Advances in Mass Spectrometry-Based Single Cell Analysis.

Siheun Lee1, Hung M Vu2, Jung-Hyun Lee2

  • 1School of Undergraduate Studies, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.

Biology
|March 29, 2023
PubMed
Summary
This summary is machine-generated.

Mass spectrometry (MS) now enables single-cell analysis, revealing cellular heterogeneity in health and disease. This review covers recent MS-based single-cell methods and their biological and medical applications.

Keywords:
mass spectrometrymass spectrometry imagingmetabolomicsproteomicssingle-cell analysis

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Single-cell analysis offers insights into cellular heterogeneity in complex biological systems.
  • Previous research primarily focused on nucleic acid analysis, with limited single-cell mass spectrometry (MS) applications.
  • MS-based single-cell analysis is emerging as a powerful tool for deeper biological understanding.

Purpose of the Study:

  • To review recent advancements in mass spectrometry (MS)-based single-cell analysis techniques.
  • To highlight the applications of these methods in biological and medical research.
  • To underscore the potential of MS for elucidating cellular mechanisms in health and disease.

Main Methods:

  • Review of current literature on MS-based single-cell analysis.
  • Discussion of technological improvements in single-cell isolation and analytical platforms.
  • Exploration of MS techniques for protein and metabolite profiling at the single-cell level.

Main Results:

  • Significant progress has been made in MS-based single-cell analysis, expanding beyond nucleic acid studies.
  • These methods provide unprecedented resolution for studying cell-to-cell variations.
  • Emerging applications demonstrate the utility of MS in understanding tissue complexity and disease states.

Conclusions:

  • MS-based single-cell analysis is a rapidly advancing field with transformative potential.
  • It offers a deeper understanding of cellular mechanisms underlying normal physiology and disease pathology.
  • Further development promises to enhance diagnostic and therapeutic strategies in medicine.