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Updated: Aug 5, 2025

Diaphragmatic Ultrasound in Adults: Image Acquisition and Interpretation
Published on: January 31, 2025
Ultrasound Imaging for Diaphragm Function in a Population of Healthy Infants: A Short Observational Report
Danilo Buonsenso1,2, Francesco Mariani1, Rosa Morello1
1Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario A. Gemelli IRCCS, Largo A. Gemelli 8, 00168 Rome, Italy.
Insights
This study establishes normal diaphragm ultrasound parameters for healthy infants up to six months old. These findings provide crucial reference data for pediatric diaphragm assessments.
Area of Science:
- Pediatric Pulmonology
- Medical Imaging
- Neonatology
Background:
- Diaphragm ultrasound is a growing diagnostic tool in adult and pediatric medicine.
- Normal diaphragm ultrasound values for healthy infants are not well-established.
- This study addresses the need for normative data in early life.
Purpose of the Study:
- To define normal diaphragm ultrasound characteristics in healthy infants during the first six months of life.
- To establish reference values for diaphragm thickness and function in neonates and young children.
Main Methods:
- Prospective observational pilot study involving healthy neonates followed for six months.
- Five assessments were conducted, measuring diaphragm thickness at end expiration (TEE) and end inspiration (TEI).
- Calculated thickening fraction (TF) using two different formulas.
Main Results:
- 37 infants were enrolled, with detailed demographic information provided.
- Normal values for TEE, TEI, and TF were determined at each assessment time point.
- The study provides the first reference data for diaphragm ultrasound parameters in healthy infants.
Conclusions:
- This research offers essential normative data for diaphragm ultrasound in healthy infants.
- The findings will support future pediatric studies investigating diaphragm physiology and pathology.
- Establishes a baseline for evaluating abnormal diaphragm function in infants.
Introduction:
Diaphragm ultrasound is increasingly used in adults, and more recently in pediatric practice. However, normal diaphragm parameters in healthy infants are unknown. This was a prospective observational pilot study aiming to define the normal diaphragm ultrasound characteristics in healthy infants during the first 6 months of life.
Methods:
We recruited healthy neonates at 7 to 15 days of life, who were followed until the sixth month of life, undergoing five assessments in different time points. The measurements included diaphragm thickness at end expiration (TEE) and at end inspiration (TEI). The thickening fraction (TF) was calculated as (TEI-TEE)/TEE and expressed as a percentage, and as (TEI-TEE)/TEI.
Results:
A total of 37 toddlers, 16 of which were females (43.2%), were enrolled. Thirty-four children (91.9%) were of Caucasian ethnicity and the median gestational age was 38.4 (35.7-40) weeks. Normal TEE, TEI, and TF have been provided for each time point.
Conclusion:
We provided new insight regarding data about thickness and thickening function in healthy children to be used for future physiologic and pathologic pediatric studies.
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