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SERPINF1 Mediates Tumor Progression and Stemness in Glioma
Lairong Song1,2, Xulei Huo1,2, Xiaojie Li1,2
1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, China.
Genes
|March 29, 2023
Summary
High SERPINF1 expression predicts poor survival in glioma patients. This protein promotes glioma cell proliferation, invasion, and stemness, suggesting SERPINF1 as a potential therapeutic target for brain tumors.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Serpin family F member 1 (SERPINF1) has known roles in various cancers, but its specific function and clinical importance in glioma remain largely unexplored.
- Glioma is a primary brain tumor with significant unmet therapeutic needs, highlighting the importance of identifying novel prognostic markers and therapeutic targets.
Purpose of the Study:
- To investigate the prognostic value and molecular mechanisms of SERPINF1 in glioma.
- To explore the association between SERPINF1 expression and glioma cell proliferation, invasion, migration, and stemness.
- To identify potential transcriptional regulators and signaling pathways involved in SERPINF1 regulation in glioma.
Main Methods:
- Analysis of SERPINF1 expression and its correlation with patient survival in three independent glioma datasets (TCGA, CGGA, GSE16011).
- In vitro experiments involving SERPINF1 knockdown to assess its effects on glioma cell proliferation, invasion, migration, and stemness.
- Single-cell level bioinformatics analysis to explore molecular functions, transcriptional regulation, and pathway associations (e.g., Notch signaling).
Main Results:
- Elevated SERPINF1 expression significantly correlated with poorer overall survival in glioma patients across all analyzed datasets (p < 0.001).
- SERPINF1 knockdown suppressed glioma cell proliferation, invasion, and migration in vitro.
- SERPINF1 was upregulated in glioma stem cells (GSCs), and its knockdown impaired GSC sphere formation.
- Bioinformatics analysis indicated a link between high SERPINF1 expression, Notch signaling activation, and potential regulation by STAT1, CREM, and NR2F2 at the single-cell level.
Conclusions:
- SERPINF1 serves as a potential prognostic biomarker for glioma patients, indicating unfavorable outcomes.
- SERPINF1 contributes to key malignant behaviors of glioma cells, including proliferation, invasion, and stemness.
- Targeting SERPINF1 may offer a promising therapeutic strategy for glioma treatment.
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