Related Experiment Video

Updated: Aug 5, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.4K

Autophagy-Related MicroRNA: Tumor miR-125b and Thyroid Cancers

Liudmila V Spirina1,2, Irina V Kovaleva1,2, Svetlana Yu Chizhevskaya1,2

  • 1Ministry of Health of Russia, Siberian State Medical University, 634050 Tomsk, Russia.

Genes
|March 29, 2023
PubMed
Abstract

Insights

Autophagy plays a role in thyroid cancer. MicroRNA-125b (miR-125b) is a potential biomarker for thyroid cancer invasion and recurrence risk, especially in patients with BRAFV600E mutations.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Autophagy, a cellular degradation process, is linked to various pathologies, including cancer.
  • Thyroid cancer is the most common endocrine malignancy.
  • Defects in autophagy are implicated in cancer development and progression.

Purpose of the Study:

  • To investigate the role of autophagy-related genes and proteins in thyroid cancer.
  • To explore the regulatory mechanisms of autophagy in thyroid cancer through bioinformatics and experimental analysis.
  • To identify potential biomarkers for thyroid cancer progression and recurrence.

Main Methods:

  • Bioinformatics analysis of autophagy-related genes and proteins in thyroid cancer.
  • Experimental verification using tissue samples from 103 thyroid cancer patients.
  • Real-time PCR detection of microRNA-125b (miR-125b) expression levels.

Main Results:

  • Bioinformatics analysis identified miR-125b as a key regulator of autophagy in thyroid cancer.
  • miR-125b expression was significantly reduced (6.75-fold) in papillary thyroid cancer (PTC) compared to benign tumors.
  • Reduced miR-125b expression (12.67-fold) was associated with BRAFV600E mutations in thyroid tumors.

Conclusions:

  • Autophagy-related proteins are involved in thyroid cancer progression.
  • Non-coding RNAs, such as miR-125b, are significant regulatory mechanisms in oncogenesis.
  • miR-125b is a potential biomarker for thyroid cancer invasion, BRAFV600E mutational status, and recurrence risk.
Keywords:
BRAFV600Eautophagyhsa-miR-125bpapillary thyroid cancerrisk of recurrencetumor invasion

More Related Videos

Spontaneous Murine Model of Anaplastic Thyroid Cancer05:39

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

1.7K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.8K
Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.1K