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Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes.

Tianyu Jiang1,2, Jingwen Song1,3, Youming Zhang1,4

  • 1Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.

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|March 29, 2023
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Bioluminescence probes, activated by luciferin-luciferase reactions, are revolutionizing optogenetics for in vivo imaging and cellular control. These bioluminescence-induced photosensory systems offer new avenues for disease research and therapeutic development.

Keywords:
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Area of Science:

  • Biotechnology
  • Optogenetics
  • Molecular Imaging

Background:

  • Bioluminescence probes are established tools for in vitro and in vivo biological process visualization.
  • Optogenetic systems increasingly leverage bioluminescence as an activation trigger.
  • Coelenterazine-type luciferin-luciferase reactions are central to these bioluminescence systems.

Purpose of the Study:

  • To review bioluminescence-induced optical probes for sensing and controlling biological processes.
  • To highlight applications and optimizations of these probes.
  • To discuss future directions in the field.

Main Methods:

  • Overview of bioluminescence-induced photosensory domain-based probes.
  • Analysis of their application in imaging, sensing, and control.
  • Review of in vitro and in vivo studies.

Main Results:

  • Bioluminescence-driven optogenetics enables precise control of cellular activities and genetic circuits.
  • These probes facilitate the study of disease mechanisms.
  • Applications span imaging, sensing, and control of biological pathways.

Conclusions:

  • Bioluminescence-induced photosensory probes are powerful tools for biological research and therapy development.
  • Further optimization and application of these optical probes are anticipated.
  • This technology holds significant promise for advancing biomedical science.