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Related Experiment Videos

Two methods compared for measuring LD-1/total LD activity in serum.

L H Bernstein, P Scinto

    Clinical Chemistry
    |May 1, 1986
    PubMed
    Summary
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    This study introduces a superior assay for lactate dehydrogenase (LD) isoenzymes 1 and 2, outperforming current methods for detecting myocardial infarction. The improved pyruvate inhibition assay offers greater accuracy and efficiency in diagnosing heart attacks.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Enzymology

    Background:

    • Lactate dehydrogenase (LD) isoenzymes are crucial biomarkers in diagnosing myocardial infarction.
    • Existing methods for LD isoenzyme analysis have limitations in accuracy and efficiency.
    • CK-MB isoenzyme evaluation often provides redundant information when LD-1 is assessed.

    Purpose of the Study:

    • To present an improved assay for lactate dehydrogenase (EC 1.1.1.27) isoenzymes 1 and 2 in serum.
    • To evaluate the utility of this assay as an index for myocardial infarction.
    • To compare the new assay's performance against existing methods, including CK-MB and immunological assays.

    Main Methods:

    • Utilized pyruvate inhibition of the H-subunit of LD at pH 7.1 for isoenzyme activity assessment.

    Related Experiment Videos

  • Correlated results with the LD-1/total LD ratio obtained via immunological assay.
  • Employed linear discriminant analysis to compare the novel method with alternative diagnostic approaches.

    • Main Results:

    • The improved LD assay demonstrated superior performance as an index for myocardial infarction compared to CK-MB activity or LD-1 activity alone or combined.
    • Results from the pyruvate inhibition assay showed good correlation with the LD-1/total LD ratio.
    • The percentage inhibition of LD activity by pyruvate exhibited a smaller population coefficient of variation (CV) for patients with acute myocardial infarction than other methods.

    Conclusions:

    • The improved lactate dehydrogenase assay, utilizing pyruvate inhibition, is a highly effective and potentially superior method for diagnosing myocardial infarction.
    • This assay offers advantages over existing methods due to its accuracy, efficiency, and smaller population CV.
    • Evaluation of CK-MB isoenzyme provides no additional diagnostic information beyond that obtained from LD-1 isoenzyme assessment.