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The Glycoprotein (GP)Ib-IX-V Complex on Platelets: GPIbα Protein Expression Is Reduced in HeartMate 3 Patients with

Kristin Klaeske1, Anneke Brade1, Sandra Eifert1

  • 1Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany.

International Journal of Molecular Sciences
|March 29, 2023
PubMed

Insights

Non-surgical bleeding is a major risk for left ventricular assist device (LVAD) patients. Reduced platelet receptor GPIbα protein expression in early bleeders may impair hemostasis and increase bleeding risk.

Area of Science:

  • Cardiovascular Medicine
  • Hematology
  • Biomedical Engineering

Background:

  • Non-surgical bleeding (NSB) is a critical complication for patients with left ventricular assist devices (LVADs).
  • High shear stress in LVADs can cause platelet dysfunction.
  • Reduced platelet receptor GPIbα expression is observed in LVAD patients with NSB.

Purpose of the Study:

  • To compare glycoprotein (GP)Ib-IX-V platelet receptor complex expression in HeartMate 3 (HM 3) patients with and without bleeding complications.
  • To investigate alterations in platelet transcriptomic profiles related to platelet damage and bleeding risk in HM 3 patients.

Main Methods:

  • Blood samples collected from HM 3 patients with NSB (n=27) and without NSB (n=55).
  • Bleeder group stratified into early (≤ 3 months) and late (> 3 months) NSB.
  • Quantified mRNA and protein expression of GPIbα, GPIX, and GPV.

Main Results:

  • No significant difference in mRNA expression of GPIbα, GPIX, and GPV between non-bleeder, early bleeder, and late bleeder groups.
  • Significantly reduced protein expression of GPIbα observed in early bleeders (≤ 3 months, p=0.04).

Conclusions:

  • Reduced platelet receptor GPIbα protein expression in early NSB post-LVAD implantation may affect platelet physiology.
  • Altered GPIbα function could impair platelet adhesion, leading to increased bleeding propensity in HM 3 patients.

Keywords:
heart failuremechanical circulatory supportnon-surgical bleedingplatelet glycoproteinventricular assist device

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