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Inhaled Nitric Oxide Promotes Angiogenesis in the Rodent Developing Brain.
Gauthier Loron1, Julien Pansiot2, Paul Olivier2
1Service de Médecine Néonatale et de Réanimation Pédiatrique, Université de Reims Champagne-Ardenne, CReSTIC, CHU Reims, 51100 Reims, France.
International Journal of Molecular Sciences
|March 29, 2023
Summary
Inhaled nitric oxide (iNO) therapy promotes blood vessel growth (angiogenesis) in the developing rat brain. This effect is mediated by the soluble guanylate cyclase/cGMP pathway, offering new insights into iNO
Area of Science:
- Neuroscience
- Developmental Biology
- Pharmacology
Background:
- Inhaled nitric oxide (iNO) is a neonatal pulmonary hypertension therapy.
- iNO exhibits potential neuroprotective properties in injured brains.
- Angiogenesis may underlie iNO's protective effects on white matter and cortex.
Purpose of the Study:
- To investigate the effect of iNO on angiogenesis in the developing brain.
- To identify potential molecular effectors mediating iNO's impact on brain development.
Main Methods:
- Utilized P14 rat pups to study brain angiogenesis.
- Examined the role of NO synthases, VEGF pathway, and angiogenic factors.
- Investigated the involvement of circulating nitrate/nitrite and the soluble guanylate cyclase/cGMP pathway.
Main Results:
- iNO significantly promoted angiogenesis in the developing white matter and cortex.
- This effect was observed during a critical developmental window.
- The pro-angiogenetic effect was linked to the soluble guanylate cyclase/cGMP pathway and thrombospondin-1.
Conclusions:
- iNO influences the developmental program of brain angiogenesis.
- Circulating nitrate/nitrite may act as NO carriers to the brain.
- The study elucidates the biological mechanisms underlying iNO's effects on the developing brain.
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