Oxygen-Releasing Hyaluronic Acid-Based Dispersion with Controlled Oxygen Delivery for Enhanced Periodontal Tissue...

Area of Science:

• Biomaterials Science
• Periodontology
• Tissue Engineering

Background:

• Periodontitis is a chronic inflammatory disease causing tooth loss, linked to anaerobic bacteria and local hypoxia impairing tissue regeneration.
• Current treatments face challenges in local oxygen delivery for effective periodontal regeneration.

Purpose of the Study:

• To develop and evaluate an oxygen (O2)-releasing hyaluronic acid (HA)-based dispersion for periodontal therapy.
• To assess the biocompatibility, angiogenic potential, and antimicrobial activity of the O2-releasing HA dispersion.

Main Methods:

• Developed an O2-releasing HA dispersion for controlled oxygen delivery.
• Assessed cell viability (fibroblasts, osteoblasts, HUVECs) and biocompatibility (CAM assay).
• Evaluated inhibition of Porphyromonas gingivalis using broth microdilution assays.

Main Results:

• The O2-releasing HA dispersion demonstrated no cytotoxicity to human cells in vitro.
• In vivo CAM assays showed enhanced angiogenesis, though not statistically significant.
• Significant inhibition of P. gingivalis growth was observed at CaO2 concentrations > 256 mg/L.

Conclusions:

• The developed O2-releasing HA dispersion is biocompatible and selectively targets P. gingivalis.
• O2-releasing biomaterials hold potential for advancing periodontal tissue regeneration therapies.