Related Experiment Video

Updated: Aug 5, 2025

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.9K

A Compendium of AR Splice Variants in Metastatic Castration-Resistant Prostate Cancer

Khrystany T Isebia1, Martijn P Lolkema1, Guido Jenster1

  • 1Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 Rotterdam, The Netherlands.

International Journal of Molecular Sciences
|March 29, 2023
PubMed

Insights

Androgen receptor splice variants (AR-Vs) drive prostate cancer resistance. This study identified recurrent AR-Vs, including AR45 and AR-V3, in metastatic castration-resistant prostate cancer (mCRPC), suggesting their potential as biomarkers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Androgen receptor (AR) alterations, including AR splice variants (AR-Vs), are implicated in prostate cancer resistance to hormonal therapies.
  • Understanding recurrent AR-Vs is crucial for developing new diagnostic and prognostic tools.

Purpose of the Study:

  • To uniformly identify recurrent AR-Vs in metastatic castration-resistant prostate cancer (mCRPC) using whole transcriptome sequencing.
  • To assess the potential diagnostic or prognostic relevance of identified AR-Vs.

Main Methods:

  • Whole transcriptome sequencing was employed to analyze AR-Vs in mCRPC samples.
  • Recurrent AR-Vs were systematically identified and characterized.

Main Results:

  • The study identified AR-V7, AR45, and AR-V3 as recurrent AR-Vs in mCRPC.
  • The presence of any AR-V was associated with higher AR expression.

Conclusions:

  • AR45 and AR-V3, in addition to AR-V7, are recurrent AR-Vs in mCRPC.
  • These AR-Vs may serve as predictive and prognostic biomarkers or indicators of high AR expression in mCRPC.
Keywords:
AR-Vs 3WTS 4androgen receptor 2prostate cancer

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.8K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.5K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.3K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.2K