Related Experiment Video
Updated: Aug 5, 2025

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
Isolation of Phosphate-Solubilizing Microorganisms and the Formulation of Biofertilizer for Sustainable Processing of
Nipuni Mayadunna1,2, Samantha C Karunarathna3,4, Suhail Asad5
1Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand.
Abstract:
As phosphorus (P) bioavailability is limited in arable lands, chemical fertilizers are being used by farmers to increase crop production. Phosphate-solubilizing microorganisms (PSMs) increase the bioavailability of sparingly soluble inorganic and organic soil phosphorus. Therefore, the current study was an effort to evaluate the phosphate-solubilizing efficiency of PSMs using tricalcium phosphate (TCP) and Eppawala rock phosphate (ERP). The efficiency of phosphate solubilization by a series of identified isolates was compared using TCP (5 g L-1) and ERP (5 g L-1) as a P source in Pikovskava's broth. Twelve microbial isolates that showed a higher efficiency in phosphate solubilization were selected for the production of the biofertilizer. The isolate F10 in ERP broth was characterized by the highest significant level of available phosphorus (896.98 ± 10.41) mg L-1, followed by F5 (Aspergillus sp.) in TCP broth 991.43 ± 1.37 mg L-1. A pot trial was carried out by using Capsicum annuum L. as the test plant in two soil conditions: sterilized soil and non-sterilized soil with six treatments and four replicates. The significantly highest plant height, leaf length, and width were shown by chili plants treated with the formulated biofertilizer. Therefore, the application of native PSMs appeared to be an efficient method of solubilizing sparingly soluble P compounds into plant-available forms.
More Related Videos
13:38Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
Published on: May 10, 2013
08:21Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
Published on: May 18, 2018
Related Concept Videos
The Phosphorus Cycle
Environmental Applications of Microorganisms
Factors Affecting Solubility
Microorganisms in Agriculture and Food industry
Metabolism of Chemolithotrophs
Bioremediation