Related Experiment Video
Updated: Aug 5, 2025
Murine Ileocolic Bowel Resection with Primary Anastomosis
Published on: October 29, 2014
Mucosa-Associated Oscillospira sp. Is Related to Intestinal Stricture and Post-Operative Disease Course in Crohn's
Shukai Zhan1, Caiguang Liu1, Jixin Meng2
1Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Abstract:
Intestinal stricture remains one of the most intractable complications in Crohn's disease (CD), and the involved mechanisms are poorly understood. Accumulating evidence suggests that the gut microbiota contributes to the pathogenesis of intestinal fibrosis. In this study, we investigated specific mucosa-associated microbiota related to intestinal strictures and their role in predicting postoperative disease course. Twenty CD patients who had undergone operative treatments were enrolled and followed up. Intestinal mucosa and full-thickness sections from stenotic and non-stenotic sites were sterilely collected. DNA extraction and bacterial 16s rRNA gene sequencing were conducted. Radiological and histological evaluations were performed to assess fibrosis. Microbial alpha diversity was significantly decreased in stenotic sites (p = 0.009). At the genus level, Lactobacillus, Oscillospira, Subdoligranulum, Hydrogenophaga, Clostridium and Allobaculum were decreased in stenotic segments (p < 0.1). The difference in Oscillospira sp. (stenotic vs. non-stenotic) was negatively correlated with the erythrocyte sedimentation rate (correlation coefficient (CC) -0.432, p = 0.057) and white blood cell count (CC -0.392, p = 0.087) and positively correlated with serum free fatty acids (CC 0.575, p < 0.05). This difference was negatively associated with intestinal fibrosis evaluated by imagological and histological methods (CC -0.511 and -0.653, p < 0.05). Furthermore, CD patients with a higher abundance of Oscillospira sp. in the residual intestine might experience longer remission periods (p < 0.05). The mucosa-associated microbiota varied between stenotic and non-stenotic sites in CD. Most notably, Oscillospira sp. was negatively correlated with intestinal fibrosis and postoperative disease course. It could be a promising biomarker to predict post-operative disease recurrence and a microbial-based therapeutic target.
Insights
Crohn's disease strictures show altered gut microbiota, with decreased *Oscillospira* linked to fibrosis. Higher *Oscillospira* abundance may predict longer remission, suggesting it as a therapeutic target for intestinal fibrosis.
Area of Science:
- Gastroenterology
- Microbiology
- Genetics
Background:
- Intestinal strictures are a major complication of Crohn's disease (CD), with poorly understood mechanisms.
- The gut microbiota is increasingly implicated in the pathogenesis of intestinal fibrosis in CD.
Purpose of the Study:
- To investigate specific mucosa-associated microbiota in CD-related intestinal strictures.
- To determine the role of these microbes in predicting the postoperative disease course and fibrosis.
Main Methods:
- Collected intestinal mucosa and tissue sections from stenotic and non-stenotic sites in 20 CD patients.
- Performed bacterial 16S rRNA gene sequencing and radiological/histological fibrosis assessment.
- Correlated microbial abundance with clinical markers and fibrosis severity.
Main Results:
- Microbial alpha diversity was significantly reduced in stenotic sites (p = 0.009).
- *Oscillospira* sp. abundance was negatively correlated with intestinal fibrosis (CC -0.511 to -0.653, p < 0.05).
- Higher *Oscillospira* sp. in residual intestine correlated with longer remission periods (p < 0.05).
Conclusions:
- Mucosa-associated microbiota differs between stenotic and non-stenotic sites in CD.
- *Oscillospira* sp. shows potential as a biomarker for predicting post-operative disease recurrence and a target for microbial-based therapies.
More Related Videos
Related Concept Videos
01:21Inflammatory Bowel Disease V: Surgical Management
Here are some common surgical interventions for IBD:
01:17Chronic Bowel Disorders: Introduction
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
01:30Esophageal Strictures-I: Introduction
Etiology
The primary cause of esophageal strictures is long-standing gastroesophageal reflux disease (GERD), accounting for about 70 to 80% of adult cases. Chronic acid reflux can lead to injury and scarring of the esophageal lining, culminating in...
01:26Esophageal Strictures-II: Clinical Features and Management
Healthcare providers should gather a comprehensive medical history and conduct a physical examination for diagnosis. If esophageal stricture is...
01:30Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
01:25Mucosal Barrier of the Stomach
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...