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Photoactivatable BODIPYs for Live-Cell PALM.

Yang Zhang1, Yeting Zheng2, Andrea Tomassini2

  • 1Program of Polymer and Color Chemistry, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606, USA.

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|March 29, 2023
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Summary

Photoactivated localization microscopy (PALM) uses engineered fluorescent probes to achieve nanoscale imaging. Borondipyrromethene (BODIPY) dyes are ideal for creating these photoactivatable probes for advanced bioimaging.

Keywords:
borondipyrromethenes (BODIPYs)fluorescence imagingphotoactivatable fluorophores (PAFs)photoactivated localization microscopy (PALM)single-molecule localization microscopy (SMLM)

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Area of Science:

  • Biophysics
  • Chemical Biology
  • Microscopy

Background:

  • Photoactivated localization microscopy (PALM) requires specialized fluorescent probes for nanoscale imaging.
  • Existing probes may not meet the stringent photochemical and photophysical demands of PALM.
  • Borondipyrromethene (BODIPY) is a versatile fluorophore with tunable properties.

Purpose of the Study:

  • To develop and characterize photoactivatable fluorescent probes based on the BODIPY scaffold for PALM.
  • To demonstrate the utility of these BODIPY-based probes for subdiffraction imaging of cellular structures.

Main Methods:

  • Synthetic modification of the BODIPY core to incorporate photolabile groups.
  • Engineering of BODIPY derivatives with photoactivatable fluorescence.
  • Incorporation of targeting ligands for specific subcellular labeling.
  • Application of developed probes in live-cell PALM imaging.

Main Results:

  • Successfully synthesized BODIPY-based photoactivatable fluorophores (PAFs).
  • Demonstrated precise single-molecule localization with nanometer precision using these PAFs.
  • Achieved subdiffraction imaging of cellular substructures in live cells via PALM.
  • Highlighted the adaptability of BODIPY scaffold for probe design.

Conclusions:

  • BODIPY derivatives are highly suitable for creating advanced photoactivatable probes for PALM.
  • These probes enable high-resolution nanoscale imaging of biological samples.
  • Photoactivatable BODIPYs represent promising tools for future bioimaging applications.