Synthesis of Cerium Oxide Nanoparticles in a Bacterial Nanocellulose Matrix and the Study of Their Oxidizing and...

A soft synthesis of nanoceria with non-stoichiometric composition (33% Ce³⁺/67% Ce⁴⁺) named CeO₂ NPs in bacterial cellulose (BC) matrix in the form of aerogel and hydrogel with controlled CeO₂ NPs content was proposed. The advantage of CeO₂ NPs synthesis in BC is the use of systemic antacid API-trisamine as a precursor, which did not destruct cellulose at room temperature and enabled a reduction in the duration of synthesis and the number of washes. Moreover, this method resulted in the subsequent uniform distribution of CeO₂ NPs in the BC matrix due to cerium (III) nitrate sorption in the BC matrix. CeO₂ NPs (0.1-50.0%) in the BC matrix had a fluorite structure with a size of 3-5 nm; the specific surface area of the composites was 233.728 m²/g. CeO₂ NPs in the BC-CeO₂ NPs composite demonstrated SOD-like activity in the processes of oxidation and reduction of cytochrome c (cyt c³⁺/cyt c²⁺), as well as epinephrine to inhibit its auto-oxidation in aqueous solutions by 33-63% relative to the control. In vitro experiments on rat blood showed a decrease in the MDA level and an increase in the activity of antioxidant defense enzymes-SOD by 24% and G6PDH by 2.0-2.5 times. Therefore, BC-CeO₂ NPs can be proposed for wound healing as antioxidant material.