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Updated: Aug 5, 2025

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
Published on: December 17, 2018
Novel Amperometric Mercury-Selective Sensor Based on Organic Chelator Ionophore
Basant Elsebai1,2, Mariana Emilia Ghica2,3, Mohammed Nooredeen Abbas4
1Water Pollution Research Department, Environmental and Climate Changes Research Institute, National Research Centre, El-Buhouth St., Dokki, Giza 12622, Egypt.
Abstract:
A novel amperometric sensor for the direct determination of toxic mercury ions, Hg2+, based on the organic chelator ionophore N, N di (2-hydroxy-5-[(4-nitrophenyl)diazenyl]benzaldehyde) benzene-1,2-diamine (NDBD), and multiwalled carbon nanotubes (MWCNT) immobilized on a glassy carbon electrode surface was developed. The parameters influencing sensor performance including the ionophore concentration, the applied potential, and electrolyte pH were optimized. The sensor response to Hg2+ was linear between 1-25 µM with a limit of detection of 60 nM. Interferences from other heavy metal ions were evaluated and the sensor showed excellent selectivity towards Hg2+. The method was successfully applied to the determination of mercury ions in milk and water samples.
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