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Bile acid metabolism in the pig
The Cornell Veterinarian
|April 1, 1986
Summary
This study tracked chenodeoxycholic acid metabolism in pigs using radiolabeling. Peak plasma levels were observed within two hours post-feeding, with a biological half-life of 6.4 days for this key bile acid.
Area of Science:
- Animal physiology
- Bile acid metabolism
- Pharmacokinetics
Background:
- Bile acids are crucial for digestion and metabolic regulation.
- Understanding bile acid kinetics is vital for metabolic research.
Purpose of the Study:
- To investigate the metabolic fate and kinetics of chenodeoxycholic acid in pigs.
- To establish a model for studying bile acid metabolism in vivo.
Main Methods:
- Surgical implantation of catheters in hepatic portal vein and anterior vena cava of pigs.
- Infusion of radiolabeled chenodeoxycholic acid (24[14C]) into the portal vein.
- Hourly monitoring of plasma radioactivity and daily collection of fecal and urine samples.
Main Results:
- Peak plasma radioactivity levels were reached within 2 hours post-feeding.
- The biological half-life of chenodeoxycholic acid was determined to be 6.4 days.
- Radioactivity was tracked in plasma, urine, and feces over multiple days.
Conclusions:
- Pigs exhibit rapid post-prandial uptake of chenodeoxycholic acid.
- The determined biological half-life provides key pharmacokinetic data for this bile acid.
- This study offers insights into porcine bile acid metabolism and enterohepatic circulation.