Preparation and Characterization of Nano-Fe₃O₄ and Its Application for C18-Functionalized Magnetic Nanomaterials Used...

A new type of magnetic nanomaterial with Fe₃O₄ as the core and organic polymer as the shell was synthesized by seed emulsion polymerization. This material not only overcomes the problem of insufficient mechanical strength of the organic polymer, it also solves the problem that Fe₃O₄ is prone to oxidation and agglomeration. In order to make the particle size of Fe₃O₄ meet the requirement of the seed, the solvothermal method was used to prepare Fe₃O₄. The effects of the reaction time, amount of solvent, pH value, and polyethylene glycol (PEG) on the particle size of Fe₃O₄ were investigated. In addition, in order to accelerate the reaction rate, the feasibility of preparing Fe₃O₄ by microwave was studied. The results showed that under the optimum conditions, the particle size of Fe₃O₄ could reach 400 nm and had good magnetic properties. After three stages of oleic acid coating, seed emulsion polymerization, and C18 modification, the obtained C18-functionalized magnetic nanomaterials were used for the preparation of the chromatographic column. Under optimal conditions, stepwise elution significantly shortened the elution time of sulfamethyldiazine, sulfamethazine, sulfamethoxypyridazine, and sulfamethoxazole while still achieving a baseline separation.