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The CRISPR/Cas9 System Delivered by Extracellular Vesicles
Xinglong Zhu1, Mengyu Gao1, Yongfeng Yang2,3
1Key Laboratory of Transplant Engineering and Immunology, Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Pharmaceutics
|March 29, 2023
Summary
Extracellular vesicles (EVs) offer a safe and effective delivery method for CRISPR/Cas9 gene editing technology. This review explores EVs
Area of Science:
- Molecular Biology
- Gene Editing Technology
- Biotechnology
Background:
- CRISPR/Cas9 gene editing holds promise for mechanistic research and disease treatment.
- Clinical applications of CRISPR/Cas9 are limited by the absence of safe and effective delivery vectors.
- Extracellular vesicles (EVs) are emerging as a promising alternative to traditional delivery systems.
Purpose of the Study:
- To review the advantages and disadvantages of CRISPR/Cas9 delivery vectors.
- To summarize the favorable traits of EVs as delivery vehicles for CRISPR/Cas9.
- To discuss EV sources, isolation, loading methods, and applications for CRISPR/Cas9 delivery.
Main Methods:
- Literature review of CRISPR/Cas9 delivery systems.
- Analysis of EV characteristics relevant to gene editing delivery.
- Discussion of current and potential applications of EV-mediated CRISPR/Cas9 delivery.
Main Results:
- EVs offer superior safety, protection, capacity, and targeting ability compared to viral vectors.
- EVs can be modified for enhanced targeting and payload delivery.
- Various EV sources, isolation techniques, and loading strategies are available for CRISPR/Cas9 delivery.
Conclusions:
- EVs represent a highly advantageous platform for in vivo CRISPR/Cas9 delivery.
- Further research is needed to optimize EV safety, capacity, quality, yield, and targeting for clinical translation.
- EVs are poised to overcome current limitations in CRISPR/Cas9 clinical applications.
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