Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

5.8K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.8K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

764
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
764
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

847
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
847
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.5K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.5K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

671
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
671

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elucidating Protein Quaternary Structure in Near-Native Solution: An AI-Assisted Hydrodynamic Approach.

Analytical chemistry·2026
Same author

Multi-Omics Analysis Identifies Paxillin as a Biomarker of Doxorubicin Resistance via Cytoskeletal Remodeling and Immune Exhaustion.

Journal of proteome research·2026
Same author

Rapid On-Site Analysis of Psychotropic Drugs in Dried Blood Spots by Capillary-in-Capillary Electrospray Ionization (DBS-CC-ESI) Miniature Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Gas-phase esterification within a miniature ion trap mass spectrometer: mechanistic insight and diagnostic markers for quinolone screening.

The Analyst·2026
Same author

Dual-Trap "Brick" Miniature Mass Spectrometer with Enhanced Sensitivity and Fragmentation Capabilities.

Analytical chemistry·2025
Same author

An Integrated Digital Microfluidic Platform with Titanium Dioxide Nanoparticles-Assisted Laser Desorption Ionization Mass Spectrometry for Microbial Analysis.

Analytical chemistry·2025
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Aug 5, 2025

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

10.8K

Smart Miniature Mass Spectrometer Enabled by Machine Learning.

Yanzuo Jiang1, Di Huang2, Hongjia Zhang2

  • 1School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.

Analytical Chemistry
|March 29, 2023
PubMed
Summary
This summary is machine-generated.

This study integrates a miniature mass spectrometer with machine learning for rapid and accurate molecule identification. The developed system achieved high accuracy in differentiating fruits and assessing strawberry freshness.

More Related Videos

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.6K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.0K

Related Experiment Videos

Last Updated: Aug 5, 2025

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

10.8K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.6K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.0K

Area of Science:

  • Analytical Chemistry
  • Computational Chemistry

Background:

  • Miniature mass spectrometers require smart features for accurate molecule identification due to lower resolution and mass drift.
  • Volatile organic compounds (VOCs) analysis is crucial for various applications, including food quality assessment.

Purpose of the Study:

  • To develop an intelligent system for rapid and accurate identification of molecules using a miniature mass spectrometer.
  • To evaluate the performance of machine learning algorithms for VOC analysis and molecule differentiation.

Main Methods:

  • A miniature mass spectrometer (Brick-V model) with a vacuum ultraviolet photoionization (VUV-PI) source was utilized.
  • Four machine learning algorithms were tested, with the multilayer perceptron (MLP) method optimized and selected.
  • The system was applied to identify ten different fruits, assess strawberry freshness, and identify 63 VOCs.

Main Results:

  • The MLP-integrated system achieved 97% average accuracy in differentiating ten fruits based on VOC patterns.
  • The system successfully discriminated strawberry freshness based on VOC profiles.
  • A rapid method for identifying 63 VOCs using molecular, fragment, and dimer ions achieved >99% prediction accuracy.

Conclusions:

  • Intelligent algorithms, particularly MLP, significantly enhance the identification capabilities of miniature mass spectrometers.
  • The developed system offers a promising tool for rapid and accurate VOC analysis in diverse applications, including food science and quality control.