Related Experiment Video

Updated: Aug 5, 2025

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

11.5K

Src inhibition induces mitotic arrest associated with chromosomal passenger complex

Song Yang1, Youguang Luo2, Mulin Yang3

  • 1Department of Cell Biology School of Basic Medical Sciences Cheeloo College of Medicine, Shandong University, Jinan, China.

Cell and Tissue Research
|March 29, 2023
PubMed

Insights

The non-receptor tyrosine kinase Src is crucial for cell division and is overactivated in cancer. Inhibiting Src disrupts mitosis by affecting the chromosomal passenger complex, offering new cancer therapy insights.

Area of Science:

  • Cell Biology
  • Cancer Biology
  • Molecular Oncology

Background:

  • The non-receptor tyrosine kinase Src is implicated in cell division, migration, adhesion, and survival.
  • Src is frequently overactivated in various cancers, promoting tumor progression and survival.
  • The precise mechanisms of Src's role in cancer and mitosis are not fully understood.

Purpose of the Study:

  • To investigate the role of Src in mitosis and its interaction with the chromosomal passenger complex.
  • To elucidate the molecular mechanisms by which Src influences cell division and chromosome segregation.
  • To provide insights into the therapeutic potential of Src inhibitors in cancer treatment.

Main Methods:

  • Analysis of Src conservation across species.
  • Microscopy to determine Src localization during mitosis.
  • Src knockdown and inhibition experiments.
  • Western blotting and co-immunoprecipitation to study protein interactions and phosphorylation.
  • Assessment of spindle formation and chromosome segregation.

Main Results:

  • Src is highly conserved and its expression increases during mitosis, localizing to the chromosomal passenger complex.
  • Src knockdown or inhibition leads to multipolar spindle formation and abnormal expression of Aurora B and INCENP.
  • Src directly interacts with and phosphorylates INCENP, disrupting chromosome arrangement and segregation.
  • Src inhibition impedes mitotic progression by inducing multipolar spindles.

Conclusions:

  • Src and the chromosomal passenger complex co-localize during mitosis.
  • Src plays a critical role in regulating mitotic progression and chromosome segregation.
  • Targeting Src may be a viable strategy for cancer therapy by disrupting cell division.
Keywords:
Chromosomal passenger complexMitosisMultipolar spindleSrc

More Related Videos

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

1.9K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.4K

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.2K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.5K