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Cytogel: A Cell-Crosslinked Thermogel.

Zhengyu Piao1, Jin Kyung Park1, Byeongmoon Jeong1

  • 1Department of Chemistry and Nanoscience, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea.

ACS Applied Materials & Interfaces
|March 29, 2023
PubMed
Summary

Researchers developed a novel thermosensitive cytogel from poly(ε-l-lysine) that forms a robust hydrogel network. This biointeractive material enhances cell proliferation and modulus, offering a new design for 3D cell culture scaffolds.

Keywords:
cell crosslinkerhydrogelreceptor−substrate bindingsol−gelthermosensitive

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Cell Biology

Background:

  • Hydrogels are versatile 3D networks with high water content, formed via chemical, physical, or biomolecular crosslinking.
  • Thermosensitive hydrogels offer tunable properties based on temperature changes.
  • Controlling hydrogel modulus is crucial for mimicking native tissue environments and improving cell viability.

Purpose of the Study:

  • To develop a thermosensitive cytogel with enhanced mechanical properties and cytocompatibility.
  • To investigate the role of substrate-receptor binding in hydrogel crosslinking and modulus control.
  • To evaluate the impact of cell type and state on the hydrogel's mechanical properties.

Main Methods:

  • Synthesis of lactobionic acid/butanoic acid-conjugated poly(ε-l-lysine) (PKLC4).
  • Characterization of thermogelation process, including sol-to-gel transition and modulus changes with temperature.
  • Incorporation of HepG2 cells into the hydrogel and assessment of cell proliferation and modulus variations.

Main Results:

  • PKLC4 exhibited thermosensitive gelation, transitioning from a sol to a gel with a modulus increase from <0.05 Pa to 1300-1360 Pa at 37 °C.
  • Incorporating HepG2 cells further increased the gel modulus to 2300-2670 Pa at 37 °C.
  • Hydrogel modulus was significantly influenced by cell type, cell population, and cell viability; cells showed improved proliferation in PKLC4 compared to PEG-PA.

Conclusions:

  • The developed PKLC4 hydrogel combines thermosensitivity and specific substrate-receptor binding for rapid, high modulus formation.
  • Substrate-receptor binding serves as an effective crosslinking strategy to precisely control hydrogel modulus.
  • This hydrogel design principle offers a promising 3D scaffold for improved cell culture and cytocompatibility.