Organic electrochemical transistor-based immuno-sensor using platinum loaded CeO₂ nanosphere-carbon nanotube and...

This work demonstrates an organic electrochemical transistor (OECT) immuno-sensor with a detection limit down to fg mL^-1. The OECT device transforms the antibody-antigen interaction signal by using the zeolitic imidazolate framework-enzyme-metal polyphenol network nanoprobe, which can produce electro-active substance (H₂O₂) through the enzyme-catalytic reaction. The produced H₂O₂ is subsequently electrochemically oxidized at the platinum loaded CeO₂ nanosphere-carbon nanotube modified gate electrode, resulting in an amplified current response of the transistor device. This immuno-sensor realizes the selective determination of vascular endothelial growth factor 165 (VEGF₁₆₅) down to the concentration of 13.6 fg mL^-1. It also shows good applicable capacity for determining the VEGF₁₆₅ that human brain microvascular endothelial cells and U251 human glioblastomas cells secreted in the cell culture medium. The ultrahigh sensitivity of the immuno-sensor is derived from excellent performances of the nanoprobe for enzyme loading and the OECT device for H₂O₂ detection. This work may provide a general way to fabricate the OECT immuno-sensing device with high performances.